KIF24 Protein (AA 1-1356) (Strep Tag)

N° du produit ABIN3135715

Détail du produit

Antigène: KIF24 (Kinesin Family Member 24 (KIF24))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1356

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette KIF24 protéine est marqué à la Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MASWLYECLC EAELAQYYPH FTALGLQKID ELAKVTMKDY SRLGVHDMND RKRLFQLIKI IKIMQEEDKA LGIPEHPLQA SSLYTKPREF RSGPRRQLHF DSPSASKDKM ANNETGSLSN FSVDEQKSTY LKVLEHMLPD DSQCQTKIRA PDASAADASM QTETNAPLFS SNYFSPQLGN CDIPVIQRVS HVSGYNYGIP HSCVRQITSE NPWTEMEKIR VCVRKRPLGV REVRRGEVNV ITVEDKETLL VHEKKEAVDL TQYILQHVFY FDEVFGEACS NQDVYLKTAH PLIQHIFNGG SATCFAYGQT GAGKTYTMIG THQNPGLYAL AAKDIFRQLK VSQSRRNLFV WISFYEIYCG QLYDLLNRRK RLFAREDSKH VVQIAGLREL QVDSVELLLQ VILKGSKERS TGATGVNADS SRSHAIIQIQ IKDSAKRTFG RISFIDLAGS ERAADARDSD RQTKMEGAEI NQSLLALKEC IRALDQEHTH TPFRQSKLTQ VLKDSFIGNA KTCMIANISP SHIATEHTLN TLRYADRVKE LKKGVKCCAS ATSQNQTSAN ASPKRIQSSP VTLPGDKCSP KKVKLGLQQS LTVAPGPTKV KAHPLASHVP NVPFTSGPKT PGKKSSSRGS PTPEWDMKAS PRKGTTRSGH SIKKGAESAP LCSEKSQIGS KIAVGWEGRA SDPGEGLLRV RLPTRGKKVQ PVQPVQKQLL SRPRLLANSH HLEATQDSKV GTPAGLAPEA WTNPILQQKE REEHLRFYHQ QFQQPPLLKQ KLNYQPLQRL LCQHRPSEGR LQSETGFPLH SNPENRDGAQ AEDLDDSDFS EDSFSHGSSQ PAMKQGSTAL ERSGSSFFLH QDREHSPEEQ AAERQQCLLF SSETDGSKKR PADSWVYSRD PIISHRRGAL SQSHSPSMVC PDWSKEEDSA SSGPSPKDNR AQKPSSSQVD FVHHQKPGEA QVSDIRLEAF TSEVPEQAEG SLSSPSPENG LSFPLSHVAV SGSPDQRDRV CTPLREVSEN RVTHTPGRVN SSTPFQEDSG EQIQMCSANA SGLMAPLTMS LLETPCHEDL SSLEQIAQDG AGYGFMAEIV GGPAAGHTVP SYDQEAALPV SSATECLWLS SSPPDNRPSG DLPALSPSPI HQHSPDKLPG REAYQTRRPI LLPENHMGSK LYDDRAEETE LGGSLTFPRK PSSNIHAGVP YSTPFLTSCT GSSNGVGRPW AQERKHPTGV SCQELVSSTD SNKPHYNEDI AWLRHRPISR CLDSDSPVVP SCSSKALRTY CPLTPEQAQQ VIIRAHKEQL DEMAELDLKE ETLMTQMDSN DFEDFVTQLD EIMALKSRCI QSLRSQLQLY LTSHRPAAAP ERTVVS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: KIF24 (Kinesin Family Member 24 (KIF24))

Autre désignation: Kif24 (KIF24 Produits)

Synonymes: C9orf48 Protein, bA571F15.4 Protein, 4933425J19Rik Protein, 9430029L23Rik Protein, RGD1307723 Protein, kinesin family member 24 Protein, KIF24 Protein, Kif24 Protein

Sujet: Kinesin-like protein KIF24,FUNCTION: Microtubule-dependent motor protein that acts as a negative regulator of ciliogenesis by mediating recruitment of CCP110 to mother centriole in cycling cells, leading to restrict nucleation of cilia at centrioles. Mediates depolymerization of microtubules of centriolar origin, possibly to suppress aberrant cilia formation. Following activation by NEK2 involved in disassembly of primary cilium during G2/M phase but does not disassemble fully formed ciliary axonemes. As cilium assembly and disassembly is proposed to coexist in a dynamic equilibrium may suppress nascent cilium assembly and, potentially, ciliar re-assembly in cells that have already disassembled their cilia ensuring the completion of cilium removal in the later stages of the cell cycle (By similarity). Plays an important role in recruiting MPHOSPH9, a negative regulator of cilia formation to the distal end of mother centriole (By similarity). {ECO:0000250|UniProtKB:Q5T7B8}.

Poids moléculaire: 150.2 kDa

UniProt: Q6NWW5