FAM178A Protein (AA 1-1278) (Strep Tag)

N° du produit ABIN3135765

Détail du produit

Antigène: FAM178A (Family with Sequence Similarity 178, Member A (FAM178A))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1278

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette FAM178A protéine est marqué à la Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MTRRCMPARP GFPSSPAPGS SPPRCHLRPG STAPAAAGKR TESPGDRKQS IIDFFKPAAK QDKHMLDSPQ KSNIKYRGNG LSITGTEQFE RKLSSPKKLK PKRMSSEESP ILEAFMKGGK EHHKDRGVHE SRRPCMSLSK YLPKGAGIYA PSSYRLPKEI KAQKKKHQSP ERRKSLFIHE SNREKNDRDR GKNSEDSRKQ ATATEGDIFK HSSRSISSRS SLSRHHPGES TLGARFQLSL ASYWREREQK KLRKEQMEQR INSENSFSEA SNLSLKSSGV GKNCKPRHEH SKHTEAVPGK SNLSTLENGH LSRKRSSSDS WELSGSKQNK FSDKRTEELC GLRPEKHKRT YHTKSKRVLS REAPRHIPSE RKVYQTHCTE DSWWCSALGR HSQGAGKTVS RGMSIASTLR LYLGRVISQL WKMDISQEKD HLQTRGNFQA LNRINSPTKE QRNSVDSDLK STKEPIIPKA RESFLEKRPD TSHQREKFIR HIALKTPGGV LRLEDIAKEP EDETDRSSAD SAPSNAGHHS SRNSDQVHSA STKETKIQKP HLPLPQEKST IKRASNLQKN KPAGSVTSKE TKLPLLSHVP SAVSSRVPLN AKNCTLPVPK KDKERSSSKE RSGHSTESSK HKEHRAKTIK AVSNESSGKN SGGSLHSEYA PPTASPPAAL EVVPSVPSPA APSDKESSGN SNAGSNALKR KFRGDFDSDE ESLGYTLESD EEEETLKSLE EIMALNFSRT PTTSGKPPAV SKGLRSQSSD MKEYAQSGTY TNTLERLVKE MEDTQRLDEL QKKLQEDIRQ GRGIKSPLRT GDQDSTDDGD GLLEEHREFL KKFSVTVDAI PDHHPGEEIF NFLNSGKIFN QYTLDLRDSG FIGESAVEKL ILKSGKTDQI FLTTQGFLTT AYHYVQCPVP VLKWLFRMMS VHTDCIVSVQ ILSTLMEITI RNDTFSDSPV WPWIPSLSDI AAVFFNMGVG FGSLFPLETL QPDFNEENLI SETQKTLGGK ESEDSPYSPV FSALPETNIL NVVKFLGLCT SIHPEGYQDG ELMLLILMLF KMSLEKELKQ IPLVDFQSLL INLMKNIRDW NTKVHELCLG INELSSHPHN LLWLVQLVPN WTSRGRQLRQ CLSLVMMSKL LDEKHEDIPN ANNLQISVLH RYLVQMKPSD LLKKMVLKKR AEQPNETIDD SLHLELEKQA YYLTYILLHL VGEVSCSHSL SSGQRKHFVL LCGALEKHVK CDIREDARLF YRTKVKDLVA RIHGKWQEII QNCRPTQGQL HDFWVPDS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: FAM178A (Family with Sequence Similarity 178, Member A (FAM178A))

Autre désignation: Slf2 (FAM178A Produits)

Synonymes: C10orf6 Protein, 3632432H19 Protein, 6030443O07Rik Protein, AU014917 Protein, FAM178A Protein, SMC5-SMC6 complex localization factor 2 Protein, SLF2 Protein, Slf2 Protein

Sujet: SMC5-SMC6 complex localization factor protein 2,FUNCTION: Plays a role in the DNA damage response (DDR) pathway by regulating postreplication repair of UV-damaged DNA and genomic stability maintenance. The SLF1-SLF2 complex acts to link RAD18 with the SMC5-SMC6 complex at replication-coupled interstrand cross-links (ICL) and DNA double-strand breaks (DSBs) sites on chromatin during DNA repair in response to stalled replication forks. Promotes the recruitment of the SMC5-SMC6 complex to DNA lesions. May play a role in SMC5-SMC6 complex recruitment for viral restriction. Forms a complex with SIMC1 and this complex is required to recruit SMC5-SMC6 complex to PML nuclear bodies and sites of viral replication. {ECO:0000250|UniProtKB:Q8IX21}.

Poids moléculaire: 144.0 kDa

UniProt: Q6P9P0