TNKS Protein (AA 1-1320) (Strep Tag)

N° du produit ABIN3135804

Détail du produit

Antigène: TNKS (Tankyrase, TRF1-Interacting Ankyrin-Related ADP-Ribose Polymerase (TNKS))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1320

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette TNKS protéine est marqué à la Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Séquence: MAASRRSQHH HHHHQQQLQP APGASAPPPP PPPPLSPGLA PGPTPASPTA GGLAPFASPR HGLALPEGDG SRDPPDRPRS PDPVDGAVCT VAAPAAVPAA SAAVGVAPTP AGGGGGGGNN SASSASSPTS SSSSSPSSPG SSLAESPEAA GVGSTATLGA GAAGLGPGVP AVSGALRELL EACRNGDVSR VKRLVDAANV NAKDMAGRKS SPLHFAAGFG RKDVVEHLLQ MGANVHARDD GGLIPLHNAC SFGHAEVVSL LLCQGADPNA RDNWNYTPLH EAAIKGKIDV CIVLLQHGAD PNIRNTDGKS ALDLADPSAK AVLTGEYKKD ELLEAARSGN EEKLMALLTP LNVNCHASDG RKSTPLHLAA GYNRVRIVQL LLQHGADVHA KDKGGLVPLH NACSYGHYEV TELLLKHGAC VNAMDLWQFT PLHEAASKNR VEVCSLLLSH GADPTLVNCH GKSAVDMAPT PELRERLTYE FKGHSLLQAA READLAKVKK TLALEIINFK QPQSHETALH CAVASLHPKR KQVAELLLRK GANVNEKNKD FMTPLHVAAE RAHNDVMEVL HKHGAKMNAL DSLGQTALHR AALAGHLQTC RLLLSYGSDP SIISLQGFTA AQMGNEAVQQ ILSESTPMRT SDVDYRLLEA SKAGDLETVK QLCSPQNVNC RDLEGRHSTP LHFAAGYNRV SVVEYLLHHG ADVHAKDKGG LVPLHNACSY GHYEVAELLV RHGASVNVAD LWKFTPLHEA AAKGKYEICK LLLKHGADPT KKNRDGNTPL DLVKEGDTDI QDLLRGDAAL LDAAKKGCLA RVQKLCTPEN INCRDTQGRN STPLHLAAGY NNLEVAEYLL EHGADVNAQD KGGLIPLHNA ASYGHVDIAA LLIKYNTCVN ATDKWAFTPL HEAAQKGRTQ LCALLLAHGA DPTMKNQEGQ TPLDLATADD IRALLIDAMP PEALPTCFKP QATVVSASLI SPASTPSCLS AASSIDNLTG PLTDLAVGGA SNAGDGAAGA ERKEGEVAGL DMNISQFLKS LGLEHLRDIF ETEQITLDVL ADMGHEELKE IGINAYGHRH KLIKGVERLL GGQQGTNPYL TFHCVNQGTI LLDLAPEDKE YQSVEEEMQS TIREHRDGGN AGGIFNRYNV IRIQKVVNKK LRERFCHRQK EVSEENHNHH NERMLFHGSP FINAIIHKGF DERHAYIGGM FGAGIYFAEN SSKSNQYVYG IGGGTGCPTH KDRSCYICHR QMLFCRVTLG KSFLQFSTMK MAHAPPGHHS VIGRPSVNGL AYAEYVIYRG EQAYPEYLIT YQIMKPEAPS QTATAAEQKT
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: TNKS (Tankyrase, TRF1-Interacting Ankyrin-Related ADP-Ribose Polymerase (TNKS))

Autre désignation: Tnks (TNKS Produits)

Synonymes: wu:fe02c12 Protein, ARTD5 Protein, PARP-5a Protein, PARP5A Protein, PARPL Protein, TIN1 Protein, TINF1 Protein, TNKS1 Protein, pART5 Protein, 4930554K12Rik Protein, AI662855 Protein, C86528 Protein, D130072O21Rik Protein, TANK1 Protein, mTNKS1 Protein, tankyrase-1 Protein, tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase b Protein, tankyrase Protein, tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase Protein, tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase 2 S homeolog Protein, tnksb Protein, TNKS Protein, Tnks Protein, tnks2.S Protein

Sujet: Poly [ADP-ribose] polymerase tankyrase-1 (EC 2.4.2.30) (ADP-ribosyltransferase diphtheria toxin-like 5) (ARTD5) (Protein poly-ADP-ribosyltransferase tankyrase-1) (EC 2.4.2.-) (TRF1-interacting ankyrin-related ADP-ribose polymerase 1) (Tankyrase I) (Tankyrase-1) (TANK1),FUNCTION: Poly-ADP-ribosyltransferase involved in various processes such as Wnt signaling pathway, telomere length and vesicle trafficking. Acts as an activator of the Wnt signaling pathway by mediating poly-ADP-ribosylation (PARsylation) of AXIN1 and AXIN2, 2 key components of the beta-catenin destruction complex: poly-ADP-ribosylated target proteins are recognized by RNF146, which mediates their ubiquitination and subsequent degradation. Also mediates PARsylation of BLZF1 and CASC3, followed by recruitment of RNF146 and subsequent ubiquitination. Mediates PARsylation of TERF1, thereby contributing to the regulation of telomere length. Involved in centrosome maturation during prometaphase by mediating PARsylation of HEPACAM2/MIKI. May also regulate vesicle trafficking and modulate the subcellular distribution of SLC2A4/GLUT4-vesicles. May be involved in spindle pole assembly through PARsylation of NUMA1. Stimulates 26S proteasome activity. {ECO:0000250|UniProtKB:O95271}.

Poids moléculaire: 140.9 kDa

UniProt: Q6PFX9