CAND1 Protein (AA 2-1230) (His tag)

N° du produit ABIN3135876

Détail du produit

Antigène: CAND1 (Cullin-Associated and Neddylation-Dissociated 1 (CAND1))

Type de proteíne: Recombinant

Attributs du protein: AA 2-1230

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette CAND1 protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Séquence: ASASYHISNL LEKMTSSDKD FRFMATNDLM TELQKDSIKL DDDSERKVVK MILRLLEDKN GEVQNLAVKC LGPLVSKVKE YQVETIVDTL CTNMLSDKEQ LRDISSIGLK TVIGELPPAS SGSALAANVC KKITGRLTSA IAKQEDVSVQ LEALDIMADM LSRQGGLLVN FHPSILTCLL PQLTSPRLAV RKRTIIALGH LVMSCGNIVF VDLIEHLLSE LSKNDSMSTT RTYIQCIAAI SRQAGHRIGE YLEKIIPLVV KFCNVDDDEL REYCIQAFES FVRRCPKEVY PHVSTIINIC LKYLTYDPNY NYDDEDEDEN AMDADGGDDD DQGSDDEYSD DDDMSWKVRR AAAKCLDAVV STRHEMLPEF YKTVSPALIA RFKEREENVK ADVFHAYLSL LKQTRPVQSW LCDPDAMEQG DTPLTMLQSQ VPNIVKALHK QMKEKSVKTR QCCFNMLTEL VNVLPGALTQ HIPVLVPGII FSLNDKSSSS NLKIDALSCL YVILCNHSPQ VFHPHVQALV PPVVACVGDP FYKITSEALL VTQQLVKVIR PLDQPSSFDA TPYIKDLFTC TIKRLKAADI DQEVKERAIS CMGQIICNLG DNLGPDLSNT LQIFLERLKN EITRLTTVKA LTLIAGSPLK IDLRPVLGEG VPILASFLRK NQRALKLGTL SALDILIKNY SDSLTAAMID AVLDELPPLI SESDMHVSQM AISFLTTLAK VYPSSLSKIS GSILNELIGL VRSPLLQGGA LSAMLDFFQA LVVTGTNNLG YMDLLRMLTG PVYSQSTALT HKQSYYSIAK CVAALTRACP KEGPAVVGQF IQDVKNSRST DSIRLLALLS LGEVGHHIDL SGQLELKSVI LEAFSSPSEE VKSAASYALG SISVGNLPEY LPFVLQEITS QPKRQYLLLH SLKEIISSAS VAGLKPYVEN IWALLLKHCE CAEEGTRNVV AECLGKLTLI DPETLLPRLK GYLISGSSYA RSSVVTAVKF TISDHPQPID PLLKNCIGDF LKTLEDPDLN VRRVALVTFN SAAHNKPSLI RDLLDSVLPH LYNETKVRKE LIREVEMGPF KHTVDDGLDI RKAAFECMYT LLDSCLDRLD IFEFLNHVED GLKDHYDIKM LTFLMLVRLS TLCPSAVLQR LDRLVEPLRA TCTTKVKANS VKQEFEKQDE LKRSAMRAVA ALLTIPEAEK SPLMSEFQSQ ISSNPELAAI FESIQKDSSS TNLESMDTS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Cand1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: CAND1 (Cullin-Associated and Neddylation-Dissociated 1 (CAND1))

Autre désignation: Cand1 (CAND1 Produits)

Synonymes: TIP120 Protein, TIP120A Protein, ATCAND1 Protein, CULLIN-ASSOCIATED AND NEDDYLATION DISSOCIATED 1 Protein, ETA2 Protein, HEMIVENATA Protein, HVE Protein, T8K22.14 Protein, T8K22_14 Protein, cullin-associated and neddylation dissociated Protein, Tip120 Protein, Tip120A Protein, fb78a05 Protein, wu:fb78a05 Protein, zgc:55729 Protein, tip120 Protein, tip120a Protein, cand2 Protein, 2310038O07Rik Protein, 6330512O03Rik Protein, AI195005 Protein, AI846556 Protein, D10Ertd516e Protein, Tp120a Protein, mKIAA0829 Protein, cullin associated and neddylation dissociated 1 Protein, cullin-associated and neddylation dissociated Protein, cullin-associated and neddylation-dissociated 1 Protein, cullin-associated and neddylation-dissociated 1 L homeolog Protein, cullin-associated NEDD8-dissociated protein 1 Protein, cullin associated and neddylation disassociated 1 Protein, CAND1 Protein, Cand1 Protein, cand1 Protein, cand1.L Protein, LOC100159824 Protein, LOC100177977 Protein

Sujet: Key assembly factor of SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complexes that promotes the exchange of the substrate-recognition F-box subunit in SCF complexes, thereby playing a key role in the cellular repertoire of SCF complexes. Acts as a F-box protein exchange factor. The exchange activity of CAND1 is coupled with cycles of neddylation conjugation: in the deneddylated state, cullin-binding CAND1 binds CUL1-RBX1, increasing dissociation of the SCF complex and promoting exchange of the F-box protein. Probably plays a similar role in other cullin-RING E3 ubiquitin ligase complexes (By similarity). {ECO:0000250}.

Poids moléculaire: 137.2 kDa Including tag.

UniProt: Q6ZQ38

Pathways: Regulation of Actin Filament Polymerization