MED26 Protein (AA 1-588) (Strep Tag)

N° du produit ABIN3135921

Détail du produit

Antigène: MED26 (Mediator Complex Subunit 26 (MED26))

Type de proteíne: Recombinant

Attributs du protein: AA 1-588

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette MED26 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MTAAPASPQQ MRDRLLQAID SQSNIRNMVA VLEVISSLER YPITKEALEE TRLGKLINDV RKKTKNEELA KRAKRLLRSW QKLIEPVHQN EVALRALAGA AGSANGGAHN CRPEMGVAGA PKSIHDLKNR NDIQRLPGQR LDRLGSRKRR GDQRDLGHPG PPHKVSKGSP DPLVPNASPL PTNGISGSPE SLPSPLDGSG HLGPDGSRLE PSDNEKHSTK IPVNAVRPRP SSPGLGKPPV PCLQTKAAQL QQLDRADESP GPPYPRGSSR CSFSPRNSRH EGSFSRHRSS YIPKGQVSSP SPWPQPPDNT QVPSPLPLAQ PPTPPVRRQE LLPNAESPVH WPEQSEGHPR LTGPACRAGF SPDSSKADSD ATSSGGSDSK KKKRYRPRDY TVNLDGQVAE AGVKPVRLKE RKLTFDPMTR QIRPLTQKEP VRADSPVPTE QLPRTELEQQ EVKASLQSPF EQTNWKELSR NEIIQSYLSR QSSLLSSSGA QTPGAHHFMA EYLKQEESSR QGARQPHVLL PLPTPTDLPG LTREVTQDDL DRIQAQQWPG VNGCEDTQGN WYDWTQCISL DPHGDDGRLN ILPYVCLD
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: MED26 (Mediator Complex Subunit 26 (MED26))

Autre désignation: Med26 (MED26 Produits)

Synonymes: med26 Protein, wu:fe06c07 Protein, wu:fi75a09 Protein, zgc:55993 Protein, CRSP7 Protein, CRSP70 Protein, 5730493L18Rik Protein, AI414941 Protein, AW495270 Protein, Crsp7 Protein, Slc35e1 Protein, crsp7 Protein, mediator of RNA polymerase II transcription subunit 26 Protein, cofactor required for Sp1 transcriptional activation, subunit 7 Protein, mediator complex subunit 26 Protein, mediator complex subunit 26 L homeolog Protein, LOC725023 Protein, crsp7 Protein, MED26 Protein, Med26 Protein, med26.L Protein

Sujet: Mediator of RNA polymerase II transcription subunit 26 (Cofactor required for Sp1 transcriptional activation subunit 7) (CRSP complex subunit 7) (Mediator complex subunit 26),FUNCTION: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional pre-initiation complex with RNA polymerase II and the general transcription factors (By similarity). {ECO:0000250}.

Poids moléculaire: 64.7 kDa

UniProt: Q7TN02

Pathways: Regulation of Lipid Metabolism by PPARalpha