DZIP3 Protein (AA 1-1204) (Strep Tag)

N° du produit ABIN3135936

Détail du produit

Antigène: DZIP3 (DAZ Interacting Protein 3, Zinc Finger (DZIP3))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1204

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette DZIP3 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MDSLAEEFFV SGNPDVEEQT KEETEIIAEK PVTQLDKQKM DISADPEPVN ALLEIKKVLN PISALPKGVF PNIEKFIQED FSFQTMQREV TTHSQTGEEI VPALTLHFLI TQLEMALRNI QASNYTAQQI NVGYYLTLLF LYGVALTERA KKEDCIEAEN KFLVMKMVIQ ESEICENFMC LVYFGRGLLR CAQKRYNGAL LEFYKSLQEI GDTDDNWFEV DPTDDEDLPT TFKDSLNNFI KTTESNIMKE TICSYLDCER SCEADILKNT NYKGFFQLMC SKSCCIYFHK ICWKKFKNLK YPGESDQSFS GQKCLKEGCP GDMVRMLQCD VPGIVKILFE VVRKDEYITI ENLGASYKNL MSLELTDTDI RPKFNLKPNT KDEVPIFKLD YNYFYHLLHI IIISGTDMVR QIFDEAMPPT LLKKELLIHK NVLEPYYNHL WTNHPLGGSW HLLYPPNKEL PQSKQFDLCL LLALIKHLNV FPAPRKGWDM EPPSSDLSKS ADILRLCKYR DILLSEILMN GLTELQFNSI WKKVSDILLR LGMKQDDLDK VKENPIENIS LDYHQLSIYL GIPVPEIIQR MLSCYQQGIT LQSITGSQRL DVEEFQNDEE DLSPPVMEYN IDVKSNTEIQ LAEINKDVAS IPSESSTESV KDLQEVKSKT KKKKRTKSNK KDKDSEDEQV SYMVEKDDQL ETEQVDVNTL STYMKTDTSD AQEDSAAEDK FCSLDELHIL DMVEQGSSGK ESTDFKETEK ERLAHQHQLY KLQYECEDYK RQLKTVTFRW QENQMLIKKK EKIIVSLNQQ VAFGINKMSK LQRQIHAKDD EIKNLKDQLS LKRSQWEMEK HNLESTVKTY LNKLNAETSR ALTAEVYFLQ CRRDFGLLHL EQTEKECLNQ LARVTHMAAS NLESLQLKAA VDSWNAIVAD VRNKIAFLRT QYNEQINKVK QGFALSTLPP VQLPPPPPSP EILIQQFLGR PLVKESFFRP ILTVPQMPAV CPGVISAAVQ PRPPLMPGIT WAMPTPIGDT VSPSASLCSE PLMINWERIT DRLKTAFPQQ TRKELTDFLQ QLKDSHGKSV SRLTFDEIVY KISQMIEPKK SESEEKSAQD GNNASPSHTA SQPNAPQDPK SAQGSATWEG DKDMDNEEEE EEPCVICHEN LSPENLSVLP CAHKFHSQCI RPWLMQQGTC PTCRLHVLQP EEFPGHPNGQ LPKI
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: DZIP3 (DAZ Interacting Protein 3, Zinc Finger (DZIP3))

Autre désignation: Dzip3 (DZIP3 Produits)

Synonymes: 2310047C04Rik Protein, 2A-HUB Protein, 6430549P11Rik Protein, A230104G20 Protein, PPP1R66 Protein, UURF2 Protein, hRUL138 Protein, RGD1563278 Protein, DAZ interacting protein 3, zinc finger Protein, DAZ interacting zinc finger protein 3 Protein, Dzip3 Protein, DZIP3 Protein

Sujet: E3 ubiquitin-protein ligase DZIP3 (EC 2.3.2.27) (DAZ-interacting protein 3 homolog) (RING-type E3 ubiquitin transferase DZIP3),FUNCTION: E3 Ubiquitin ligase proteins mediate ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Able to specifically bind RNA. {ECO:0000250|UniProtKB:Q86Y13}.

Poids moléculaire: 138.0 kDa

UniProt: Q7TPV2