IKAP/p150 Protein (AA 1-1333) (Strep Tag)

N° du produit ABIN3135967

Détail du produit

Antigène: IKAP/p150 (ELP1) (Elongator Complex Protein 1 (ELP1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1333

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette IKAP/p150 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MRNLKLHRTL EFRDIQAPGK PQCFCLRAEQ GTVLIGSERG LTEVDPVRRE VKTEISLVAE GFLPEDGSGC IVGIQDLLDQ ESVCVATASG DVIVCNLSTQ QLECVGSVAS GISVMSWSPD QELLLLATAQ QTLIMMTKDF EVIAEEQIHQ DDFGEGKFVT VGWGSKQTQF HGSEGRPTAF PVQLPENALP WDDRRPHITW RGDGQYFAVS VVCRQTEARK IRVWNREFAL QSTSESVPGL GPALAWKPSG SLIASTQDKP NQQDVVFFEK NGLLHGHFTL PFLKDEVKVN DLLWNADSSV LAIWLEDLPK EDSSTLKSYV QLWTVGNYHW YLKQSLPFST TGKNQIVSLL WDPVTPCRLH VLCTGWRYLC CDWHWTTDRS SGNSANDLAN VAVIDGNRVL VTVFRQTVVP PPMCTYRLLI PHPVNQVIFS AHLGNDLAVL DASNQISVYK CGDKPNMDST VKLGAVGGNG FKVPLTTPHL EKRYSIQFGN NEEEEEEEVN ALQLSFLTWV EDDTFLAISY SHSSSQSIIH HLTVTHSEVD EEQGQLDVSS SVTVDGVVIG LCCCSKTKSL AVQLADGQVL KYLWESPSLA VEPWKNSEGI PVRFVHPCTQ MEVATIGGEE CVLGLTDRCR FFINDTEVAS NITSFAVCDD FLLVTTHSHT CQVFSLSGAS LKMLQAALSG SHEASGEILR KVERGSRIVT VVPQDTKLIL QMPRGNLEVV HHRALVLAQI RKWLDKLMFK EAFECMRKLR INLNLIHDHN PKVFLENVET FVKQIDSVNH INLFFTELRE EDVTKTMYPP PITKSVQVST HPDGKKLDLI CDAMRAAMEA INPRKFCLSI LTSHVKKTTP ELEIVLQKVQ ELQGNLPFDP ESVSVEEALK YLLLLVDVNE LFNHSLGTYD FNLVLMVAEK SQKDPKEYLP FLNTLKKMET NYQRFTIDKY LKRYEKALGH LSKCGPEYFT ECLNLIKDKN LYKEALKLYR PDSPQYQAVS MAYGEHLMQE HLYEPAGLVF ARCGAQEKAL EAFLACGSWQ QALCVAAQLQ MSKDKVAGLA RTLAGKLVEQ RKHSEAATVL EQYAQDYEEA VLLLLEGSAW EEALRLVYKY DRVDIIETSV KPSILEAQKN YMDFLDSETA TFIRHKNRLQ VVRALRRQAP QVHVDHEVAH GPESDLFSET SSIMSGSEMS GRYSHSNSRI SARSSKNRRK AERKKHSLKE GSPLEGLALL EALSEVVQSV EKLKDEVRAI LKVLFLFEFE EQAKELQRAF ESTLQLMERA VPEIWTPAGQ QSSTTPVLGP SSTANSITAS YQQQKTCVPA LDAGVYMPPK MDPRSQWKLS LLE
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: IKAP/p150 (ELP1) (Elongator Complex Protein 1 (ELP1))

Autre désignation: Elp1 (ELP1 Produits)

Synonymes: DYS Protein, ELP1 Protein, FD Protein, IKAP Protein, IKI3 Protein, TOT1 Protein, IKBKAP Protein, DKFZp469N1415 Protein, 3110040G09Rik Protein, 6030413P05 Protein, C78473 Protein, Elp1 Protein, Ikbkap Protein, elongator complex protein 1 Protein, inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase complex-associated protein Protein, ielongator complex protein 1 S homeolog Protein, inhibitor of kappa light polypeptide enhancer in B cells, kinase complex-associated protein Protein, ELP1 Protein, Elp1 Protein, IKBKAP Protein, elp1.S Protein, elp1 Protein, Ikbkap Protein

Sujet: Elongator complex protein 1 (ELP1) (IkappaB kinase complex-associated protein) (IKK complex-associated protein),FUNCTION: Component of the elongator complex which is required for multiple tRNA modifications, including mcm5U (5-methoxycarbonylmethyl uridine), mcm5s2U (5-methoxycarbonylmethyl-2-thiouridine), and ncm5U (5-carbamoylmethyl uridine) (By similarity). The elongator complex catalyzes the formation of carboxymethyluridine in the wobble base at position 34 in tRNAs (PubMed:23717213). Regulates the migration and branching of projection neurons in the developing cerebral cortex, through a process depending on alpha-tubulin acetylation (PubMed:22854966). ELP1 binds to tRNA, mediating interaction of the elongator complex with tRNA (By similarity). May act as a scaffold protein that assembles active IKK-MAP3K14 complexes (IKKA, IKKB and MAP3K14/NIK) (By similarity). {ECO:0000250|UniProtKB:O95163, ECO:0000250|UniProtKB:Q06706, ECO:0000269|PubMed:22854966}.

Poids moléculaire: 149.6 kDa

UniProt: Q7TT37