CLASP1 Protein (AA 1-1535) (Strep Tag)

N° du produit ABIN3136001

Détail du produit

Antigène: CLASP1 (Cytoplasmic Linker Associated Protein 1 (CLASP1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1535

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette CLASP1 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MEPRMESCLA QVLQKDVGKR LQVGQELIDY FSDRQKSADL EHDQTLLDKL VDGLATSWVN SSNYKVVLLG MDILSALVTR LQDRFKAQIG TVLPSLIDRL GDAKDSVREQ DQTLLLKIMD QAANPQYVWD RMLGGFKHKN FRTREGICLC LIATLNASGA QTLTLSKIVP HICNLLGDPN SQVRDAAINS LVEIYRHVGE RVRADLSKKG LPQSRLNVIF TKFDEVQKSG NMIQSANEKN FDDEDSVDGN RPSSASSSSS KAPSSSRRNV NLGTTRRLMS SSLGSKSSAA KEGAGAVDEE DFIKAFDDVP VVQIYSSRDL EESINKIREI LSDDKHDWEQ RVNALKKIRS LLLAGAAEYD NFFQHLRLLD GAFKLSAKDL RSQVVREACI TLGHLSSVLG NKFDHGAEAI MPTIFNLIPN SAKIMATSGV VAVRLIIRHT HIPRLIPVIT SNCTSKSVAV RRRCFEFLDL LLQEWQTHSL ERHISVLAET IKKGIHDADS EARIEARKCY WGFHSHFSRE AEHLYHTLES SYQKALQSHL KNSDSIVSLP QSDRSSSSSQ ESLNRPLSAK RSPTGSTASR GSTVSTKSVS TTGSLQRSRS DIDVNAAASA KSKVSSSSGS PAFSSAAALP PGSYASLGRI RTRRQSSGST TNVASTPDSR GRSRAKVVSQ SQRSRSANPA GAGSRSSSPG KLLGSGLAGG SSRGPPVTPS SEKRSKIPRS QGCSRETSPN RIGLARSSRI PRPSMSQGCS RDTSRESSRD TSPARGFTPL DRFGLGQSGR IPGSVNAMRV LSTSTDLEAA VADALKKPVR RRYEPYGMYS DDDANSDASS VCSERSYGSR NGGIPHYLRQ TEDVAEVLNH CASSNWSERK EGLLGLQNLL KSQRTLSRVE LKRLCEIFTR MFADPHSKRV FSMFLETLVD FIIIHKDDLQ DWLFVLLTQL LKKMGADLLG SVQAKVQKAL DVTRDSFPFD QQFNILMRFI VDQTQTPNLK VKVAILKYIE SLARQMDPTD FVNSSETRLA VSRIITWTTE PKSSDVRKAA QIVLISLFEL NTPEFTMLLG ALPKTFQDGA TKLLHNHLKN SSNTGVGSPS NTIGRTPSRH PSSRTSPLTS PTNCSHGGLS PSRLWGWSAD GLSKPPPPFS QPNSIPTAPS HKTLRRSYSP SMLDYDTENL NSEEIYSSLR GVTEAIEKFS FRSQEDLNEP IKRDGKKDCD IVSRDGGAAS PATEGRGGSE IEGGRMALDN KTSLLNTQPP RAFPGPRARE YNPYPYSDTI NTYDKTALKE AVFDDDMEQL RDVPIDHSDL VADLLKELSN HNERVEERKG ALLELLKITR EDSLGVWEEH FKTILLLLLE TLGDKDHSIR ALALRVLREI LRNQPARFKN YAELTIMKTL EAHKDSHKEV VRAAEEAAST LASSIHPEQC IKVLCPIIQT ADYPINLAAI KMQTKVVERI TKESLLQLLV DIIPGLLQGY DNTESSVRKA SVFCLVAIYS VIGEDLKPHL AQLTGSKMKL LNLYIKRAQT TNSNSSSSSD VSTHS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: CLASP1 (Cytoplasmic Linker Associated Protein 1 (CLASP1))

Autre désignation: Clasp1 (CLASP1 Produits)

Synonymes: Xorbit Protein, clasp1 Protein, clasp1b Protein, mast1 Protein, CLASP1 Protein, 1700030C23Rik Protein, 5730583A19Rik Protein, B130045P17Rik Protein, mKIAA0622 Protein, MAST1 Protein, cytoplasmic linker associated protein 1 S homeolog Protein, cytoplasmic linker associated protein 1b Protein, cytoplasmic linker associated protein 1 Protein, CLIP-associating protein 1 Protein, CLIP associating protein 1 Protein, clasp1.S Protein, clasp1b Protein, CLASP1 Protein, clasp1 Protein, LOC100078792 Protein, Clasp1 Protein

Sujet: CLIP-associating protein 1 (Cytoplasmic linker-associated protein 1),FUNCTION: Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle (By similarity). {ECO:0000250}.

Poids moléculaire: 169.2 kDa

UniProt: Q80TV8

Pathways: Dynamique des Microtubules, M Phase, Maintenance of Protein Location