DNAJC6 Protein (AA 1-938) (Strep Tag)

N° du produit ABIN3136004

Détail du produit

Antigène: DNAJC6 (DnaJ (Hsp40) Homolog, Subfamily C, Member 6 (DNAJC6))

Type de proteíne: Recombinant

Attributs du protein: AA 1-938

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette DNAJC6 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MTNPKSGVAE SAGLACSRAA AGENRMKDSE NKGASSPDME PSYGGGLFDM VKGGAGRLFS NLKDNLKDTL KDTSSRVIQS VSSYTKGDLD FTYVTSRIIV MSFPVDSVDI GFRNQVDDIR SFLDSRHLDH YTVYNLSPKS YRTAKFHSRV SECSWPIRQA PSLHNLFAVC RNMYNWLLQN PKNVCVVHCL DGRAASSILV GAMFIFCNLY STPGPAVRLL YAKRPGIGLS PSHRRYLGYM CDLLADKPYR PHFKPLTIKA ITVSPVPFFN KQRNGCRPYC DVLIGETKIY STCTDFERMK EYRVQDGKIF IPLNITVQGD VIVSMYHLRS TIGSRLQAKV TNTQIFQLQF HSGFIPLDTT VLKFTKPELD ACDVPEKYPQ LFQVTLDIEV QPQDKVIDLT PPWEHYCTKD VNPSILFSSQ QEHQDTLALG GQAPADLPPD HPRNLGQGGF FASLCWQDQK SEKSRCEEDH AALVNQESEQ SDDELLTLSS PHGNAEGDKP HGAKKPGKKQ QEPAAPPPPE EVDLLGLEGS DVSTNFSSLA APPSNSELLS DLFGGVGATG PAQAGQAGVE DVFHPSGPVS AQSTPRRTAT SASASPTLRV GEGATFDPFG APAKPPGQDL LGSFLNTSSA SSDPFLQPTR SPSPTVHASS TPAVNIQPDI AGGWDWHTKP GGFGMGSKSA ATSPTGSSHG TPTHQSKPQT LDPFADLGTL GSSSFASKPT TPTGLGGGFP PLSSPQKASP QPMGGGWQQP AGYNWQQTQS KPQSSMPHSS PQNRPNYNVS FSAMPAGQSE RGKGSTNLEG KQKAADFEDL LSSQGFNAHK DKKGPRTIAE MRKEEMAKEM DPEKLKILEW IEGKERNIRA LLSTMHTVLW AGETKWKPVG MADLVTPEQV KKVYRRAVLV VHPDKATGQP YEQYAKMIFM ELNDAWSEFE NQGQKPLY
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: DNAJC6 (DnaJ (Hsp40) Homolog, Subfamily C, Member 6 (DNAJC6))

Autre désignation: Dnajc6 (DNAJC6 Produits)

Synonymes: DJC6 Protein, 2810027M23Rik Protein, mKIAA0473 Protein, DnaJ heat shock protein family (Hsp40) member C6 Protein, DNAJC6 Protein, Dnajc6 Protein

Sujet: Auxilin (EC 3.1.3.-) (DnaJ homolog subfamily C member 6),FUNCTION: May act as a protein phosphatase and/or a lipid phosphatase. Co-chaperone that recruits HSPA8/HSC70 to clathrin-coated vesicles (CCVs) and promotes the ATP-dependent dissociation of clathrin from CCVs and participates in clathrin-mediated endocytosis of synaptic vesicles and their recycling and also in intracellular trafficking (PubMed:20160091). Firstly, binds tightly to the clathrin cages, at a ratio of one DNAJC6 per clathrin triskelion. The HSPA8:ATP complex then binds to the clathrin-auxilin cage, initially at a ratio of one HSPA8 per triskelion leading to ATP hydrolysis stimulation and causing a conformational change in the HSPA8. This cycle is repeated three times to drive to a complex containing the clathrin-auxilin cage associated to three HSPA8:ADP complex. The ATP hydrolysis of the third HSPA8:ATP complex leads to a concerted dismantling of the cage into component triskelia. Then, dissociates from the released triskelia and be recycled to initiate another cycle of HSPA8's recruitment. Also acts during the early steps of clathrin-coated vesicle (CCV) formation through its interaction with the GTP bound form of DNM1 (By similarity). {ECO:0000250|UniProtKB:Q27974, ECO:0000269|PubMed:20160091}.

Poids moléculaire: 102.3 kDa

UniProt: Q80TZ3