AKNA Protein (AA 1-1404) (Strep Tag)

N° du produit ABIN3136045

Détail du produit

Antigène: AKNA (AT-Hook Transcription Factor (AKNA))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1404

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette AKNA protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MASSGAKAQW VGPSLGQGPR RRRWAWAEEQ DTDGRRDQGW GNSQSLPEAP SPELLEDFRR AQEHLPPLEW DPDMQDSEES SGEETEADDA SSPEGSTVPL PWLSRSNQQL DMSEEELDEA SGRPEVDLAG ESCTELECED QGDSSPPPPG QGPAKGWVTF IKQGSNYRPS EHLEAQPSVE HSRTKSWSSG TVSLRQPSDS LGSTWEGDTE VPQPSILPKA LPQSPCHNFP HPGDRNGGDV APATPTEFRD SLAAPAQNAE CSAGTWGEET TSLPSSRPED QTWKRTKTSP KPLPSRFTGS VSPLSTRLGA IKKVVPQHKQ GATLAGHSSS QAPKYGRGRR LNYPLPDFSK VGPRVRFPKD ENYRPPKSRG HNRQQGSTRP LIFKSPAEIV RDVLLSSGEA SLAKESSLAH TITRVPQEFQ TPEQATELVH QLQEDYHKLL TKYAEAENTI DQLRLGAKVH LYSDPPQPSQ SFCSGSMPQG SKVLSFSIPQ PRVAEWWPDP AQDPQASEAT GWPFPRTDLS PSSSPGVATP GRLPQSQGIA TDQPSTGQTQ ALTSQASRLL AKVQSFEELV LAGHLPPQDQ IKSLEQLRAA HMALEAEYLQ ACREELLDPQ LDASQGSPRT LNLCRELEAE IYHLGQRLEE LQDHMDQTQR ETEPCRPDLQ DSTPTMSFLP QSAHLSMPSG PVSLPDGQTY QEPATTTTTS PGSSCTLPIN KKLSLSIKTE ESPRGLPVPL RDRTLQVEQD FHGLLERYLS VKSLPEALRD EDEDDLEEEE EEQDHQGPLE VDSPATAPGK TEAVRVPPGE RPTQAEESHR DATQEDEEQM GPMKSPDFRP SMARDTYTPV LDTAEVAQRG TKAMVSHQSS LTSLEESRPS ELLPRKALLR AGGPHTEEPW MVSPETDSGF VGSETSIVSP FTQTPEHRLS HVSTSGPSAQ HLTASVPGDR TSHPKARGLM VPRRATETGI PRSRTQQHFS SLSSPGRGAQ SCHLEETSVA KIAVPRSEFK RQKQISKQLL PSGRTSPDSA PAPTAASTPH GSAESTANLL LNRTERDQAI KDLQAEVSRL RLQLEDSLHR PHPDGPACVA SAFNHSTQTQ EKLGSSPSWG PHYGSKSTER LSREPNGVEP AEPMGRRRAR SSSVPRDVPR LYLSSESESP APRLSSEKSR TFEEHPEAAQ WGTRPQSSSK RRERVSFRGQ YTGQEYHILS PKAILKDSGT PSCPHCHPIR TQDTGSAVSR DTTRGSSAAD TLRCALCGEV KSSAEADGSS SGPSEKNTPK KPSTPILKRK NRQTGSPVRM APGLWYLAAA PPAPAPPALA YISSAPIMPY LPPTVYYAAP APTSAQTASP QPARGPRRTR HSVQLGLNDL EELQAALREA AQAAENVRST TRQLSRSLSA DLRHARSLRG SCLF
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: AKNA (AT-Hook Transcription Factor (AKNA))

Autre désignation: Akna (AKNA Produits)

Synonymes: CASKIN1 Protein, AI597013 Protein, mKIAA1968 Protein, AT-hook transcription factor Protein, AT-hook-containing transcription factor Protein, Akna Protein, AKNA Protein, LOC539274 Protein

Sujet: Microtubule organization protein AKNA (AT-hook-containing transcription factor),FUNCTION: Centrosomal protein that plays a key role in cell delamination by regulating microtubule organization (PubMed:30787442). Required for the delamination and retention of neural stem cells from the subventricular zone during neurogenesis (PubMed:30787442). Also regulates the epithelial-to-mesenchymal transition in other epithelial cells (PubMed:30787442). Acts by increasing centrosomal microtubule nucleation and recruiting nucleation factors and minus-end stabilizers, thereby destabilizing microtubules at the adherens junctions and mediating constriction of the apical endfoot (PubMed:30787442). In addition, may also act as a transcription factor that specifically activates the expression of the CD40 receptor and its ligand CD40L/CD154, two cell surface molecules on lymphocytes that are critical for antigen-dependent-B-cell development (By similarity). Binds to A/T-rich promoters (By similarity). It is unclear how it can both act as a microtubule organizer and as a transcription factor, additional evidences are required to reconcile these two apparently contradictory functions (Probable). {ECO:0000250|UniProtKB:Q7Z591, ECO:0000269|PubMed:30787442, ECO:0000305}.

Poids moléculaire: 153.1 kDa

UniProt: Q80VW7