ANKFY1 Protein (AA 2-1169) (His tag)

N° du produit ABIN3136096

Détail du produit

Antigène: ANKFY1 (Ankyrin Repeat and FYVE Domain Containing 1 (ANKFY1))

Type de proteíne: Recombinant

Attributs du protein: AA 2-1169

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette ANKFY1 protéine est marqué à la His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), ELISA

Séquence: AEEEVAKLEK HLMLLRQEYV KLQKKLAETE KRCTLLAAQA NKENSNESFI SRLLAIVAGL YEQEQYSDLK IKVGDRHISA HKFVLAARSD SWSLANLSST EEIDLSDANP EVTMTMLRWI YTDELEFRED DVFLTELMKL ANRFQLQLLR ERCEKGVMSL VNVRNCIRFY QTAEELNAST LMNYCAEIIA SHWDDLRKED FSSLSAQLLY KMIKSKTEYP LHKAIKVERE DVVFLYLIEM DSQLPGKLNE TDHNGDLALD LALSRRLESI ATTLVSHKAD VDMVDKNGWS LLHKGIQRGD LFASTFLIKN GALVNAATAG AQETPLHLVA LYSPKKYSAD VMSEMAQIAE ALLQAGANPN MQDSKGRTPL HLSIMARNDC VFSQLLQCKQ LDLELKDHEG STALWLAVQY ITVSSDQSVN PFEDLPVVNG TSFDENSFAA RLIQRGSNTD APDVMTGNCL LQRAAGAGNE AAALFLATSG AHANHRNKWG ETPLHTACRH GLANLTAELL QQGANPNLQT EEALPVPKES PVLMSSADSI YLQTPLHMAI AYNHPDVVSV ILEQKANALH ATNNLQIIPD FSLKDSRDQT VLGLALWTGM HTIAAQLLGS GASINDTMSD GQTLLHMAIQ RQDSKSALFL LEHQADINVR TQDGETALQL AIKHQLPLVV DAICTRGADM SVPDEKGNPP LWLALASNLE DIASTLVRHG CDATCWGPGP SGCLQTLLHR AVDENNESTA CFLIRSGCDV NSPRQPGTNG EGEEEARDGQ TPLHLAASWG LEETVQCLLE FGANVNAQDA EGRTPVHVAI SNQHSVIIQL LISHPNIELS VRDRQGLTPF ACAMTYKNNK AAEAILKRES GAAEQVDNKG RNFLHVAVQN SDIESVLFLI SVQANVNSRV QDASKLTPLH LAVQAGSEII VRNLLLAGAK VNELTKHRQT ALHLAAQQDL PTICSVLLEN GVDFAAVDEN GNNALHLAVM HGRLNNIRAL LTECTVDAEA FNLRGQSPLH ILGQYGKENA AAIFDLFLEC MPEYPLDKPD AEGNTVLLLA YMKGNANLCR AIVRSGVRLG VNNNQGVNIF NYQVATKQLL FRLLDMLSKE PPWCDGSNCY ECTAKFGVTT RKHHCRHCGR LLCHKCSTKE IPIIKFDLNK PVRVCNICFD VLTLGGVS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Ankfy1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ANKFY1 (Ankyrin Repeat and FYVE Domain Containing 1 (ANKFY1))

Autre désignation: Ankfy1 (ANKFY1 Produits)

Synonymes: ANKHZN Protein, BTBD23 Protein, ZFYVE14 Protein, Ankhzn Protein, mKIAA1255 Protein, ankyrin repeat and FYVE domain containing 1 Protein, ANKFY1 Protein, Ankfy1 Protein

Sujet: Proposed effector of Rab5. Binds to phosphatidylinositol 3-phosphate (PI(3)P). Involved in homotypic early endosome fusion and to a lesser extend in heterotypic fusion of chlathrin-coated vesicles with early endosomes. Required for correct endosomal localization. Involved in the internalization and trafficking of activated tyrosine kinase receptors such as PDGFRB. Regulates the subcellular localization of the retromer complex in a EHD1-dependent manner. Involved in endosome-to-Golgi transport and biosynthetic transport to late endosomes and lysosomes indicative for a regulation of retromer complex-mediated retrograde transport (By similarity). Involved in macropinocytosis, the function is dependent on Rab5-GTP. {ECO:0000250|UniProtKB:Q9P2R3, ECO:0000269|PubMed:15328530}.

Poids moléculaire: 129.5 kDa Including tag.

UniProt: Q810B6