PSD4 Protein (AA 1-1005) (Strep Tag)

N° du produit ABIN3136219

Détail du produit

Antigène: PSD4 (Pleckstrin and Sec7 Domain Containing 4 (PSD4))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1005

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette PSD4 protéine est marqué à la Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MMGDHLRPME CLNICLEDNL QPCPEAYPSE IHGHPGPSEP CQEHTCPFDP PESARPDAPH GNSGVESTHL ENCLVQVQAR QASASLRSLE DNSSLGSPQQ NQSSSTQVVF WAGILQAQMC VLDLEEELEK TEGLRAELRC CIPPPSKDLL GDEGLSPSRP EEDEDSGDDS SGPEEENQTW PREKIPGSSL EWGAEEDSIF FDNPLFLESP CSDTSTEGEC FSWGYPNSHP DMKTWHQSPQ TLDSPLQKGT GLWRQENELD LGSDTADHGG CSTPPFPVPS YKMHPCLALG STEGDPTVPP DQEGETSCED DLGHGSPKAP FVDHELIQES DNFEFDLRPA TTHPVQPWGS QTSQSLSDLT QPILEDLQRE DPSRSQETLI SQNRGERDAG CFQEPVFCTL APWGSQTSLL EPNCPESEGR GSGPQPSPVS SQDSSPRVLL HSPKWPQDAS HLLQKDRSEL SSLKEEETEE VPSLRQEAEC EDTSRSEDAS ANQHHVHLAS AEGLPESPMP QAQSPEEGWR PSSSREKLAN DIRNDKGAWN LALRLYQLNG FRKSEVAAHL RKNNDFSRAV AEAYLSFFQF EGQSLDRALR GFLQALVLSG ETQERERILY QFSKRFHYCN PGAFPSVDSV HTLTCAIMLL NTDLHGQNIG KSMSCQEFVT NLNGLQDGRN FPKELLKALY WSIRSEKLEW AIDEEDADRP EKDQPSPSAG KISSPFLQMA QDPTMPTYKQ GILARKMHHI ADGKKTPWGK RGWKMFHTLL RGMVLYFLKG EGQWLDGESL VGHMVDEPVG VHHSLASPAT HYTKKPHVFQ LRTADWRLYL FQAPTAKEMA SWIARINLAA ATHSAPPFPA AVGSQRRFVR PILPMSPVQS SLEEQHRSHE NCLDAASDDL LDLQRNLPER RGRSRELEEY RLRKEYLEHE KTRYETYVQL LVARLHFPLG DLALWEDQLG KETDGSQEPR PSLKKSHSSP SLHQEEAPTT AKVKRNISER RTYRKIIPKR NRNQL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: PSD4 (Pleckstrin and Sec7 Domain Containing 4 (PSD4))

Autre désignation: Psd4 (PSD4 Produits)

Synonymes: EFA6B Protein, TIC Protein, BC046518 Protein, pleckstrin and Sec7 domain containing 4 Protein, PSD4 Protein, Psd4 Protein

Sujet: PH and SEC7 domain-containing protein 4 (Exchange factor for ADP-ribosylation factor guanine nucleotide factor 6 B) (Exchange factor for ARF6 B) (Pleckstrin homology and SEC7 domain-containing protein 4),FUNCTION: Guanine nucleotide exchange factor for ARF6 and ARL14/ARF7. Through ARL14 activation, controls the movement of MHC class II-containing vesicles along the actin cytoskeleton in dendritic cells. Involved in membrane recycling. Interacts with several phosphatidylinositol phosphate species, including phosphatidylinositol 3,4-bisphosphate, phosphatidylinositol 3,5-bisphosphate and phosphatidylinositol 4,5-bisphosphate (By similarity). {ECO:0000250}.

Poids moléculaire: 112.7 kDa

UniProt: Q8BLR5