ABI1 Protein (AA 2-481) (His tag)

N° du produit ABIN3136405

Détail du produit

Antigène: ABI1 (Abl-Interactor 1 (ABI1))

Type de proteíne: Recombinant

Attributs du protein: AA 2-481

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette ABI1 protéine est marqué à la His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: AELQMLLEEE IPSGKRALIE SYQNLTRVAD YCENNYIQAT DKRKALEETK AYTTQSLASV AYQINALANN VLQLLDIQAS QLRRMESSIN HISQTVDIHK EKVARREIGI LTTNKNTSRT HKIIAPANME RPVRYIRKPI DYTVLDDVGH GVKWLKAKHG NNQPARTGTL SRTNPPTQKP PSPPVSGRGT LGRNTPYKTL EPVKPPTVPN DYMTSPARLG SQHSPGRTAS LNQRPRTHSG SSGGSGSREN SGSSSIGIPI AVPTPSPPTA GPAAPGAAPG SQYGTMTRQI SRHNSTTSST SSGGYRRTPS VAAQFSAQPH VNGGPLYSQN SISVAPPPPP MPQLTPQIPL TGFVARVQEN IADSPTPPPP PPPDDIPMFD DSPPPPPPPP VDYEDEEAAV VQYSDPYADG DPAWAPKNYI EKVVAIYDYT KDKDDELSFK EGAIIYVIKK NDDGWFEGVC NRVTGLFPGN YVESIMHYTD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Abi1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ABI1 (Abl-Interactor 1 (ABI1))

Autre désignation: Abi1 (ABI1 Produits)

Synonymes: ABI-1 Protein, ABLBP4 Protein, E3B1 Protein, NAP1BP Protein, SSH3BP Protein, SSH3BP1 Protein, NAP1 Protein, Ssh3bp1 Protein, E3b1 Protein, abi1 Protein, wu:fc66g09 Protein, wu:fk56a10 Protein, zgc:73174 Protein, MGC84356 Protein, ABI1 Protein, abi-1 Protein, e3b1 Protein, nap1bp Protein, ssh3bp Protein, ssh3bp1 Protein, wu:fe48a10 Protein, zgc:153534 Protein, DKFZp469P1319 Protein, abl interactor 1 Protein, abl-interactor 1 Protein, abl-interactor 1a Protein, abl interactor 1 S homeolog Protein, abl-interactor 1b Protein, Abl-interactor 1 Protein, Abl interactor 1 Protein, ABI1 Protein, Abi1 Protein, abi1a Protein, abi1.S Protein, abi1 Protein, abi1b Protein, Bm1_44660 Protein, LOAG_04356 Protein

Sujet: May act in negative regulation of cell growth and transformation by interacting with nonreceptor tyrosine kinases ABL1 and/or ABL2. In vitro, at least isoform 2 and isoform 4 suppress the transforming activity of Abelson murine leukemia virus (v-Abl) after overexpression in fibroblasts. May play a role in regulation EGF-induced Erk pathway activation. Involved in cytoskeletal reorganization and EGFR signaling. Together with EPS8 participates in transduction of signals from Ras to Rac. In vitro, a trimeric complex of ABI1, EPS8 and SOS1 exhibits Rac specific guanine nucleotide exchange factor (GEF) activity and ABI1 seems to act as an adapter in the complex. Regulates ABL1/c-Abl-mediated phosphorylation of ENAH. Recruits WASF1 to lamellipodia and there seems to regulate WASF1 protein level. In brain, seems to regulate the dendritic outgrowth and branching as well as to determine the shape and number of synaptic contacts of developing neurons. {ECO:0000269|PubMed:10499589, ECO:0000269|PubMed:11526477, ECO:0000269|PubMed:12672821, ECO:0000269|PubMed:15143189, ECO:0000269|PubMed:15558031, ECO:0000269|PubMed:7590237}.

Poids moléculaire: 53.1 kDa Including tag.

UniProt: Q8CBW3

Pathways: Signalisation RTK, Response to Water Deprivation, ER-Nucleus Signaling