NEMF Protein (AA 1-1064) (Strep Tag)

N° du produit ABIN3136412

Détail du produit

Antigène: NEMF (SDCCAG1) (serologically Defined Colon Cancer Antigen 1 (SDCCAG1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1064

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette NEMF protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MKSRFSTVDL RAVLAELNAS LLGMRVNNVY DVDNKTYLIR LQKPDFKATL LLESGIRIHT TEFEWPKNMM PSSFAMKCRK HLKSRRLVSA KQLGVDRIVD FQFGSDEAAY HLIIELYDRG NIVLTDYEYL ILNILRFRTD EADDVKFAVR ERYPIDHARA AEPLLTLERL TEVIAAAPKG EVLKRVLNPL LPYGPALIEH CLIESGFSGN AKVDEKLESK DIEKILVCVQ RAEDYLRKTS NFNGKGYIIQ KREAKPSLDA DKPAEDILTY EEFHPFLFSQ HLQCPYIEFE SFDKAVDEFY SKIEGQKIDL KALQQEKQAL KKLDNVRKDH ENRLEALQQA QEIDKLKGEL IEMNLQIVDR AIQVVRSALA NQIDWTEIGV IVKEAQAQGD PVACAIKELK LQTNHVTMLL RNPYLLSEEE DGDGDASIEN SDAEAPKGKK KKQKNKQLQK PQKNKPLLVD VDLSLSAYAN AKKYYDHKRY AAKKTQRTVE AAEKAFKSAE KKTKQTLKEV QTVTSIQKAR KVYWFEKFLW FISSENYLII GGRDQQQNEI IVKRYLTPGD IYVHADLHGA TSCVIKNPTG EPIPPRTLTE AGTMALCYSA AWDARVITSA WWVYHHQVSK TAPTGEYLTT GSFMIRGKKN FLPPSYLMMG FSFLFKVDES CVWRHRGERK VRVQDEDMET LTSCTSELMA EEMEQLEGGD SSEEETEELH GMPGDVELMT QVDQEDIAVH SGRDELSSED GEAKAVTKDQ EPIGEMKEEE EDTFEYPDTT IDLSHLQSQR PLQKLAPREE SLNSNDSKSQ GRRHLSAKER REMKKKKLPC ESGDLEVIEE KDKERESAVH TEAYQNTSKN VAAGQPMKRG QKSKMKKMKE KYKDQDDEDR ELIMKLLASA GSNKEEKGKK GKKGKPKDEP VKKPPQKPRG GQRVLDVVKE PPSLQVLAHD LQDLAVDDPH DDKEEHDLDQ QGNEENLFDS LTGQPHPEDV LMFAIPICAP YTIMTNYKYK VKLTPGVQKK GKAAKTALNS FMHSKEATAR EKDLFRSVKD TDLSRNIPGK VKVSAPNLLH VKRK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: NEMF (SDCCAG1) (serologically Defined Colon Cancer Antigen 1 (SDCCAG1))

Autre désignation: Nemf (SDCCAG1 Produits)

Synonymes: NY-CO-1 Protein, SDCCAG1 Protein, 1500011I12Rik Protein, 4933405E14Rik Protein, Sdccag1 Protein, nuclear export mediator factor Protein, NEMF Protein, Nemf Protein

Sujet: Ribosome quality control complex subunit NEMF (Nuclear export mediator factor),FUNCTION: Key component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates the extraction of incompletely synthesized nascent chains from stalled ribosomes as well as their ubiquitin-mediated proteasomal degradation (PubMed:33406423). Thereby, frees 60S subunit ribosomes from the stalled translation complex and prevents the accumulation of nascent polypeptide chains that are potentially toxic for the cell (PubMed:33406423). Within the RQC complex, NEMF specifically binds stalled 60S ribosomal subunits by recognizing an exposed, nascent chain-conjugated tRNA moiety and promotes the recruitment of LTN1 to stalled 60S subunits (By similarity). Following binding to stalled 60S ribosomal subunits, NEMF mediates CAT tailing by recruiting alanine-charged tRNA to the A-site and directing the elongation of stalled nascent chains independently of mRNA or 40S subunits, leading to non-templated C-terminal alanine extensions (CAT tails) (PubMed:33406423). Mainly recruits alanine-charged tRNAs, but can also other amino acid-charged tRNAs (By similarity). CAT tailing is required to promote ubiquitination of stalled nascent chains by different E3 ubiquitin-protein ligases (By similarity). In the canonical RQC pathway (RQC-L), CAT tailing facilitates LTN1-dependent ubiquitination by exposing lysine residues that would otherwise remain buried in the ribosomal exit tunnel (By similarity). In the alternative RQC pathway (RQC-C) CAT tailing creates an C-degron mainly composed of alanine that is recognized by the CRL2(KLHDC10) and RCHY1/PIRH2 E3 ligases, leading to ubiquitination and degradation of stalled nascent chains (By similarity). NEMF may also indirectly play a role in nuclear export (By similarity). {ECO:0000250|UniProtKB:O60524, ECO:0000250|UniProtKB:Q12532, ECO:0000269|PubMed:33406423}.

Poids moléculaire: 121.2 kDa

UniProt: Q8CCP0