RAB11FIP3 Protein (AA 1-1047) (Strep Tag)

N° du produit ABIN3136469

Détail du produit

Antigène: RAB11FIP3 (RAB11 Family Interacting Protein 3 (Class II) (RAB11FIP3))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1047

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette RAB11FIP3 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MELCQPTSLS DHDQPASGPQ RGVMGLVGPD APRGWSEEPE EHAQLQRWPE GPNAPICWPE EVEEPHAPSR WAKEPNAPRC SSQEPDESCH LAEELEESDS PRCWPQEPDT PCHLAKELEE PDAPRCLPQE PDTPCYLAKE LEEPNIPRCW PQEPDVPCHL AKELEEPDAP RCWPQEPDAF CHLLKEVEEP DALRCWLQGP DAPCHLAKEL EDLDSPRCWP QEPDESCHLA KELEEPDAPC HLAKELEEPD APRCWPQEPD VPCLLAKKWE ESDAPCLLTE ELEEPDALHC WPQESEAPCL LAKELEEPDA SHSCPQEADT GCLSAKEPEE PDVSHLWQGV PDAPCLLVKE PEEADALHCC WPEESEEPDA LNPPCFWANE PDEPDPSRCW SEEPQVLCLW PEEQNTKRCW QEEPDAPCFW PEDREEPIVS CLQFKEPEKP KVRSSWPEEL EDCCPTRGLP LEPLLADGEL LQACPGPPSD PGPALSLPSE PGTAQEEGAR LRAVFDALDR DGDGFVRIED FIQFATVYGA EQVKDLTQYL DPSGLGVISF EDFYQGIVAI RNGDPDGQLY SVEPVQDEET PACADEFDDF VTYEANEVTD SAYMGSESTY SECETFTDED TSTLVHPELQ PEGDVDSAGG SGVPSECLDT MEEPDHGALL LLPGRSRPHS QAVVMVIGSE EHFEDYGEGN EAELSPETLC DGDGEDPAFL TPSPAKRLSS RKVARYLHQS GTLTMEALED PPPEPVECPE EDIADKVIFL ERRVSELEKD SAAAGEQHGR LRQENLQLVH RANALEEQLK EQEFRAQEKV LEETRKQKEL LCKMEREKSI EIENLQARLQ QLDEENSELR SCTPCLKANI ERLEEEKQKM LDEIEELTQR LSEEQENKRK MGDRLSHERH QFQRDKEATQ ELIEDLRKQL EHLQLLRLEV EQRRGRSSSL GLQEYNSRAR ESELEQEVRR LKQDNRNLKE QNDELNGQII TLSIQGAKSL FSTSFSESLA AEISSVSRDE LMEAIQKQEE INFRLQDYID RIIVAILETN PSILEVK
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: RAB11FIP3 (RAB11 Family Interacting Protein 3 (Class II) (RAB11FIP3))

Autre désignation: Rab11fip3 (RAB11FIP3 Produits)

Synonymes: LRRGT00043 Protein, RGD1308952 Protein, MNDPK Protein, RAB11FIP3 Protein, CART1 Protein, Rab11-FIP3 Protein, Cart1 Protein, D030060O14Rik Protein, mKIAA0665 Protein, si:ch211-153c20.4 Protein, zC153C20.4 Protein, RAB11 family interacting protein 3 Protein, NME/NM23 nucleoside diphosphate kinase 4 Protein, RAB11 family interacting protein 3 (class II) Protein, Rab11fip3 Protein, NME4 Protein, RAB11FIP3 Protein, rab11fip3 Protein

Sujet: Rab11 family-interacting protein 3 (FIP3) (FIP3-Rab11) (Rab11-FIP3),FUNCTION: Downstream effector molecule for Rab11 GTPase which is involved in endocytic trafficking, cytokinesis and intracellular ciliogenesis by participating in membrane delivery (PubMed:18685082). Recruited by Rab11 to endosomes where it links Rab11 to dynein motor complex. The functional Rab11-RAB11FIP3-dynein complex regulates the movement of peripheral sorting endosomes (SE) along microtubule tracks toward the microtubule organizing center/centrosome, generating the endocytic recycling compartment (ERC) during interphase of cell cycle. Facilitates the interaction between dynein and dynactin and activates dynein processivity (By similarity). Binding with ASAP1 is needed to regulate the pericentrosomal localization of recycling endosomes (PubMed:18685082). The Rab11-RAB11FIP3 complex is also implicated in the transport during telophase of vesicles derived from recycling endosomes to the cleavage furrow via centrosome-anchored microtubules, where the vesicles function to deliver membrane during late cytokinesis and abscission. The recruitment of Rab11-RAB11FIP3-containing endosomes to the cleavage furrow and tethering to the midbody is co-mediated by RAB11FIP3 interaction with ARF6-exocyst and RACGAP1-MKLP1 tethering complexes. Also involved in the Rab11-Rabin8-Rab8 ciliogenesis cascade by facilitating the orderly assembly of a ciliary targeting complex containing Rab11, ASAP1, Rabin8/RAB3IP, RAB11FIP3 and ARF4, which directs preciliary vesicle trafficking to mother centriole and ciliogenesis initiation. Also promotes the activity of Rab11 and ASAP1 in the ARF4-dependent Golgi-to-cilia transport of the sensory receptor rhodopsin. Competes with WDR44 for binding to Rab11, which controls intracellular ciliogenesis pathway. May play a role in breast cancer cell motility by regulating actin cytoskeleton (By similarity). {ECO:0000250|UniProtKB:O75154, ECO:0000269|PubMed:18685082}.

Poids moléculaire: 118.5 kDa

UniProt: Q8CHD8