DIS3L2 Protein (AA 1-870) (His tag)
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- Antigène Voir toutes DIS3L2 Protéines
- DIS3L2 (DIS3-Like Exonuclease 2 (DIS3L2))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-870
- Origine
- Souris
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Source
- Cellules d'insectes
- Purification/Conjugué
- Cette DIS3L2 protéine est marqué à la His tag.
- Application
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Séquence
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MNHPDYKLNL RSPGTPRGVS SVVGPSAVGA SPGDKKSKNK SMRGKKKSIF ETYMSKEDVS EGLKRGTLIQ GVLRINPKKF HEAFIPSPDG DRDIFIDGVV ARNRALNGDL VVVKLLPEDQ WKAVKPESND KEIEATYEAD IPEEGCGHHP LQQSRKGWSG PDVIIEAQFD DSDSEDRHGN TSGLVDGVKK LSISTPDRGK EDSSTPVMKD ENTPIPQDTR GLSEKSLQKS AKVVYILEKK HSRAATGILK LLADKNSDLF KKYALFSPSD HRVPRIYVPL KDCPQDFMTR PKDFANTLFI CRIIDWKEDC NFALGQLAKS LGQAGEIEPE TEGILTEYGV DFSDFSSEVL ECLPQSLPWT IPPDEVGKRR DLRKDCIFTI DPSTARDLDD ALACRRLTDG TFEVGVHIAD VSYFVPEGSS LDKVAAERAT SVYLVQKVVP MLPRLLCEEL CSLNPMTDKL TFSVIWKLTP EGKILEEWFG RTIIRSCTKL SYDHAQSMIE NPTEKIPEEE LPPISPEHSV EEVHQAVLNL HSIAKQLRRQ RFVDGALRLD QLKLAFTLDH ETGLPQGCHI YEYRDSNKLV EEFMLLANMA VAHKIFRTFP EQALLRRHPP PQTKMLSDLV EFCDQMGLPM DVSSAGALNK SLTKTFGDDK YSLARKEVLT NMYSRPMQMA LYFCSGMLQD QEQFRHYALN VPLYTHFTSP IRRFADVIVH RLLAAALGYS EQPDVEPDTL QKQADHCNDR RMASKRVQEL SIGLFFAVLV KESGPLESEA MVMGVLNQAF DVLVLRFGVQ KRIYCNALAL RSYSFQKVGK KPELTLVWEP DDLEEEPTQQ VITIFSLVDV VLQAEATALK YSAILKRPGL EKASDEEPED
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Attributs du produit
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Dis3l2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Protein is endotoxin free.
- Classe de qualité
- Crystallography grade
- Top Product
- Discover our top product DIS3L2 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Commentaires
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- DIS3L2 (DIS3-Like Exonuclease 2 (DIS3L2))
- Autre désignation
- Dis3l2 (DIS3L2 Produits)
- Synonymes
- RGD1560168 Protein, DIS3L2 Protein, DKFZp469O0522 Protein, FAM6A Protein, PRLMNS Protein, hDIS3L2 Protein, 4930429A22Rik Protein, 8030493P09Rik Protein, DIS3-like 3'-5' exoribonuclease 2 Protein, DIS3 like 3'-5' exoribonuclease 2 Protein, DIS3-like exonuclease 2 Protein, Dis3l2 Protein, DIS3L2 Protein, LOC100344728 Protein
- Sujet
- 3'-5'-exoribonuclease that specifically recognizes RNAs polyuridylated at their 3' end and mediates their degradation. Component of an exosome-independent RNA degradation pathway that mediates degradation of both mRNAs and miRNAs that have been polyuridylated by a terminal uridylyltransferase, such as ZCCHC11/TUT4. Mediates degradation of cytoplasmic mRNAs that have been deadenylated and subsequently uridylated at their 3'. Mediates degradation of uridylated pre-let-7 miRNAs, contributing to the maintenance of embryonic stem (ES) cells. Essential for correct mitosis, and negatively regulates cell proliferation. {ECO:0000255|HAMAP-Rule:MF_03045, ECO:0000269|PubMed:23594738, ECO:0000269|PubMed:25119025}.
- Poids moléculaire
- 98.7 kDa Including tag.
- UniProt
- Q8CI75
- Pathways
- Stem Cell Maintenance
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