MYO3A Protein (AA 1-1613) (Strep Tag)

N° du produit ABIN3136580

Détail du produit

Antigène: MYO3A (Myosin IIIA (MYO3A))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1613

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette MYO3A protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MLPLIGKTII FDNFPDPSDT WEIIETIGKG TYGKVFKVLN KKSGQKAAVK ILDPIHDIDE EIEAEYNILR TLSDHPNVVR FYGIYFKKDK INGDKLWLVL ELCNGGSVTD LVKGFLKRGE RMSEPVIAYI LHEALMGLQH LHSNKTIHRD VKGNNILLTT EGGVKLVDFG VSAQLSSTRH RLNTSVGTPF WMAPEVIACE QQLDTTYDAR CDTWSLGITA IELGDGDPPL AELHPMRALF KIPRNPPPKL RQPELWSAEF NDFISKCLTK DYEKRPTVSD LLKHKFITQI EGKDVILQKQ LMEFIDIHQC LGSTEKARHE RIHTKKGNLN RSLISSLKDV DDLATLDVLD EPTASPHLHP CHSRDQIHIH VADILIALNP FQSLGIYSPK LSRLYIGAKR TANPPHIFAM ADLGYQSMVT YNADQCIVIS GESGAGKTES AHLLVQQLTV LGKANNRTLQ EKILQMNNLV EAFGNACTII NDNSSRFGKY LEMKFTSSGA VVGAQISEYL LEKSRVIHQA MGEKNFHIFY YIYGGLAEKK KLALYKSPEH KPPRYLQNDN LRTVQDMMNN SFYKSQYELI EQCFKVIGFT MEQLASVYSV LAAILNVGNI EFSSVATEYQ MDKSYICNHT ALENCASLLC IQADELQEAL TSHCVVTRGE TIIRPNTVEK AADVRDAMAK TLYGRLFSCI VNCINSLLKH DTSPSGDEEL NIGILDIFGF ENFKRNSFEQ LCINIANEQI QYYFNQHVFA WEQNEYLNED VDARVIEYED NRPLLDMFLQ KPMGLLSLLD EESRFPKATD QTLIEKFEDN LKSQYFWRPK RMELSFGIHH YAGKVLYSAS GFLAKNRDTF PTDIVLPLRS SENSVIRQLV NHPLTKTGNL PLSKTKNIVN YQMWNSEKST NLTKGETRDV TCHACETTNV KTQTVSSYFR YSLMDLLSKM VVGQPHFVRC IKPNNERQAR KYDKEKVLLQ LRCTGILETA RIRRLGYSHR ILFANFIKRY YILCYKSSEE PPVSPDTCAA ILEKAGLDNW ALGKTKVFLK YYHVEQLNLM RKEATNKLVL IQASVRAFLG ARRYQELQQK RKSSAVIIQS AARGHLVRKQ RKEIVDMKNT AVTTIQTSDQ EFDYKKNFEN TRESFVKKQT ENAVPTNESN TSTPNNKESP SAGKTAPFIA ESKATNVESN NRRYHTQKKM SNVYAEGQNQ ELYIVEDTWA EVSPRQKYVQ DLEESRKMRK EEKGDAVIQS YCQWYTEGSN FEESKATCLE GRETWERTSC PGLWLTEEIY LRKTLDPTLS QKSVYQNADG KEKEHKVSVV TQNAPLGNLE RDYHLLGFLG EEDTGPVPQA QEEHKAVSIH SKYQSSKKKQ QLGKDRLAPP FKNQKILSSS TEVAKTTHNV YPCPTKQEGV HHSKMVDERD SKMASKKEAW DLAMFSRQIS KLSEEYFILQ KNLNEIILAQ QLKPFYLGIY RHKPINRHVS THQYLSGVSK GEEPKILRPP RRPRKPKTLN NPEDSTYYYL LHKSTQEEKR RPGKDSQGKL LGLEDFYYKE FLPTHYGPKA HSSNAREWKA LKEPQAQPIE SNERCWTTSE NESLEEERIS ANPYDYRRLL RKTSQRQRLV QQL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: MYO3A (Myosin IIIA (MYO3A))

Autre désignation: Myo3a (MYO3A Produits)

Synonymes: MYO3A Protein, RGD1560083 Protein, DFNB30 Protein, 9030416P08Rik Protein, myosin IIIA Protein, myo3a Protein, MYO3A Protein, Myo3a Protein, CpipJ_CPIJ005840 Protein

Sujet: Myosin-IIIa (EC 2.7.11.1),FUNCTION: Probable actin-based motor with a protein kinase activity. Probably plays a role in vision and hearing (By similarity). Required for normal cochlear hair bundle development and hearing. Plays an important role in the early steps of cochlear hair bundle morphogenesis. Influences the number and lengths of stereocilia to be produced and limits the growth of microvilli within the forming auditory hair bundles thereby contributing to the architecture of the hair bundle, including its staircase pattern (PubMed:26754646). Involved in the elongation of actin in stereocilia tips by transporting the actin regulatory factor ESPN to the plus ends of actin filaments (PubMed:19287378). {ECO:0000250|UniProtKB:Q8NEV4, ECO:0000269|PubMed:19287378, ECO:0000269|PubMed:26754646}.

Poids moléculaire: 184.7 kDa

UniProt: Q8K3H5

Pathways: Sensory Perception of Sound, Phototransduction