SF3A1 Protein (AA 2-791) (His tag)

N° du produit ABIN3136618

Détail du produit

Antigène: SF3A1 (Splicing Factor 3a, Subunit 1 (SF3A1))

Type de proteíne: Recombinant

Attributs du protein: AA 2-791

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette SF3A1 protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Séquence: QAGPVQAVPP PPPVATESKQ PIEEEASSKE DPTPSKPVVG IIYPPPEVRN IVDKTASFVA RNGPEFEARI RQNEINNPKF NFLNPNDPYH AYYRHKVSEF KEGKAQEPSA AIPKVMQQQQ QATQQQLPQK VQAQVIQETI VPKEPPPEFE FIADPPSISA FDLDVVKLTA QFVARNGRQF LTQLMQKEQR NYQFDFLRPQ HSLFNYFTKL VEQYTKILIP PKGLFSKLKK EAENPREVLD QVCYRVEWAK FQERERKKEE EEKEKERVAY AQIDWHDFVV VETVDFQPNE QGNFPPPTTP EELGARILIQ ERYEKFGESE EVEMEVESDE EDQEKAEETP SQLDQDTQVQ DMDEGSDDEE EGQKVPPPPE TPMPPPLPPT PDQVIVRKDY DPKASKPLPP APAPDEYLVS PITGEKIPAS KMQEHMRIGL LDPRWLEQRD RSIREKQSDD EVYAPGLDIE SSLKQLAERR TDIFGVEETA IGKKIGEEEI QKPEEKVTWD GHSGSMARTQ QAAQANITLQ EQIEAIHKAK GLVPEDDTKE KIGPSKPNEI PQQPPPPSSA TNIPSSAPPI TSVPRPPAMP PPVRTTVVSA VPVMPRPPMA SVVRLPPGSV IAPMPPIIHA PRINVVPMPP AAPPIMAPRP PPMIVPTAFV PAPPVAPVPA PAPMPPVHPP PPMEDEPPSK KLKTEDSLMP EEEFLRRNKG PVSIKVQVPN MQDKTEWKLN GQGLVFTLPL TDQVSVIKVK IHEATGMPAG KQKLQYEGIF IKDSNSLAYY NMASGAVIHL ALKERGGRKK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Sf3a1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: SF3A1 (Splicing Factor 3a, Subunit 1 (SF3A1))

Autre désignation: Sf3a1 (SF3A1 Produits)

Synonymes: Dmel\\CG16941 Protein, SF3a1 Protein, SF3a120 Protein, DDBDRAFT_0168938 Protein, DDBDRAFT_0233180 Protein, DDB_0168938 Protein, DDB_0233180 Protein, 1200014H24Rik Protein, 5930416L09Rik Protein, AI159724 Protein, si:dz150f13.2 Protein, wu:fc38e01 Protein, wu:fc50a11 Protein, wu:fj37c05 Protein, zgc:65786 Protein, PRP21 Protein, PRPF21 Protein, SAP114 Protein, SF3A120 Protein, CG16941 gene product from transcript CG16941-RA Protein, SWAP/Surp domain-containing protein Protein, splicing factor 3a, subunit 1 Protein, splicing factor 3a subunit 1 Protein, CG16941 Protein, sf3a1 Protein, spl1 Protein, Sf3a1 Protein, SF3A1 Protein

Sujet: Subunit of the splicing factor SF3A required for 'A' complex assembly formed by the stable binding of U2 snRNP to the branchpoint sequence (BPS) in pre-mRNA. Sequence independent binding of SF3A/SF3B complex upstream of the branch site is essential, it may anchor U2 snRNP to the pre-mRNA. May also be involved in the assembly of the 'E' complex (By similarity). {ECO:0000250}.

Poids moléculaire: 89.4 kDa Including tag.

UniProt: Q8K4Z5

Pathways: Ribonucleoprotein Complex Subunit Organization