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MCMBP Protein (AA 1-642) (His tag)

Crystallography grade MCMBP Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, Crys, SDS, WB
N° du produit ABIN3136655
  • Antigène Voir toutes MCMBP Protéines
    MCMBP (Minichromosome Maintenance Complex Binding Protein (MCMBP))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-642
    Origine
    • 1
    • 1
    Souris
    Source
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette MCMBP protéine est marqué à la His tag.
    Application
    ELISA, Crystallization (Crys), SDS-PAGE (SDS), Western Blotting (WB)
    Séquence
    MPCGEDWLSH PLGIVQGFFA QNGVNPDWEK KVIEYFKEKL KENNAPKWVP SLNEVPLHYL KPNSFVKFRC MIQDMFDPEF YMGIYETVNQ NTKARVLHFG KYRDVAECGP QQELDLSSPR STTSERQTFY CVPVPGESSW VKEAYVNANQ ARVSPSTSYT PSRHKRSYED DEDMDLQPSK QKEQHPGSRQ AGGLGGLHWC GEPKRLETEA SSGQQLNTLN LSSPFDLNFP LPGEKGPACL VKVYEDWDCF KVNDVLELYG VLSVDPVLSV LNSEERDASA LLDPMECTDM AEEQRVHSPP ASLVPRIHVI LAQKLQHINP LLPTCLNKEE SRSCQFVSNF MSELSPVRAE LLGFLTHALL GDSLAAEYLI LHLISTVYTR RDVLPLGKFT VNLSGCPQNS TFTEHLYRII QHLVPASFRL QMTIENMNQL KLIPHKDYTA NRLVSGLLQL PNNTSLVIDE TLLEQGQLDT PGVHNVTALS NLITWQKVDY DFSYHQMEFP CNINVLITSE GRSLLPADCQ IHLQPQLIPP NMEEYMNGLL SAVLPSVLNK FRIYLTLLRF LDYNLSDDIT KAVEDDFVEM RKDDPQSITA DDLHQLLVVA RFLSLSVGQT TLSRERWLRA KQLELSRKAR LQQQKSVNGN EL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Mcmbp Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product MCMBP Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    MCMBP (Minichromosome Maintenance Complex Binding Protein (MCMBP))
    Autre désignation
    Mcmbp (MCMBP Produits)
    Synonymes
    1110007A13Rik Protein, AU017382 Protein, BC025641 Protein, MCM-BP Protein, C26H10ORF119 Protein, C10orf119 Protein, c10orf119 Protein, flj13081 Protein, cb136 Protein, sb:cb136 Protein, wu:fi19h07 Protein, zgc:55981 Protein, Mcmbp Protein, minichromosome maintenance complex binding protein Protein, minichromosome maintenance complex binding protein L homeolog Protein, Mcmbp Protein, MCMBP Protein, mcmbp.L Protein, mcmbp Protein
    Sujet
    Associated component of the MCM complex that acts as a regulator of DNA replication. Binds to the MCM complex during late S phase and promotes the disassembly of the MCM complex from chromatin, thereby acting as a key regulator of pre-replication complex (pre-RC) unloading from replicated DNA. Can dissociate the MCM complex without addition of ATP, probably acts by destabilizing interactions of each individual subunits of the MCM complex. Required for sister chromatid cohesion (By similarity). {ECO:0000250}.
    Poids moléculaire
    73.8 kDa Including tag.
    UniProt
    Q8R3C0
    Pathways
    Chromatin Binding
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