DHX36 Protein (AA 1-1001) (Strep Tag)

N° du produit ABIN3136749

Détail du produit

Antigène: DHX36 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 36 (DHX36))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1001

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette DHX36 protéine est marqué à la Strep Tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: MSYDYHQSWS RDGGPRGSGQ GSSGGGGGGS RGSGGGGGGR GGRGRHPAHL KGREIGLWYA KKQTQKNKEA ERQERAVVHM DERREEQIVQ LLNSVQAKTD KDSEAQISWF APEDHGYGTE VSSEKKINSE KKLDNQEKKL LNQEKKTFRI TDKSYIDRDS EYLLQENEPN LSLDQHLLED LQRKKTDPRY IEMQRFRKKL PSYGMQKELV NLINNHQVTV ISGETGCGKT TQVTQFILDN YIERGKGSAC RIVCTQPRRI SAISVAERVA TERAESCGNG NSTGYQIRLQ SRLPRKQGSI LYCTTGIILQ WLQSDSRLSS VSHIVLDEIH ERNLQSDVLM TVIKDLLHFR SDLKVILMSA TLNAEKFSEY FGNCPMIHIP GFTFPVVEYL LEDIIEKIRY VPDQKEHRSQ FKRGFMQGHV NRQEKEEKEA IYKERWPAYI KELRTRYSAS TVDVLQMMDD DKVDLNLIAA LIRYIVLEEE DGAILVFLPG WDNISTLHDL LMSQVMFKSD KFLIIPLHSL MPTVNQTQVF KKTPPGVRKI VIATNIAETS ITIDDVVYVI DGGKIKETHF DTQNNISTMS AEWVSKANAK QRKGRAGRVQ PGHCYHLYNG LRASLLDDYQ LPEILRTPLE ELCLQIKILR LGGIAYFLSR LMDPPSNEAV VLSIKHLMEL SALDKQEELT PLGVHLARLP VEPHIGKMIL FGALFCCLDP VLTIAASLSF KDPFVIPLGK EKIADARRKE LAKETRSDHL TVVNAFEGWE EAKRRGFRYE KDYCWEYFLS SNTLQMLHNM KGQFAEHLLG AGFVSSRSPK DPKANINSDN EKIIKAVICA GLYPKVAKIR LNLGKKRKMV KVHTKSDGLV SIHPKSVNVE QTDFHYNWLI YHLKMRTSSI YLYDCTEVSP YCLLFFGGDI SIQKDKDQEI IAVDEWIVFQ SPERIAHLVK GLRKELDSLL QEKIESPHPV DWDDTKSRDC AVLSAILDLI KTQEKATPRN LPPRSQDGYY S
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: DHX36 (DEAH (Asp-Glu-Ala-His) Box Polypeptide 36 (DHX36))

Autre désignation: Dhx36 (DHX36 Produits)

Synonymes: DHX57 Protein, DDX36 Protein, G4R1 Protein, MLEL1 Protein, RHAU Protein, 2810407E23Rik Protein, AI452301 Protein, AU022184 Protein, Ddx36 Protein, mKIAA1488 Protein, DEAH-box helicase 36 Protein, ATP-dependent RNA helicase DHX36 Protein, DEAH (Asp-Glu-Ala-His) box polypeptide 36 Protein, Dhx36 Protein, DHX36 Protein, dhx36 Protein, LOC100456222 Protein, LOC100633205 Protein

Sujet: ATP-dependent DNA/RNA helicase DHX36 (EC 3.6.4.13) (DEAD/H box polypeptide 36) (DEAH box protein 36) (MLE-like protein 1) (RNA helicase associated with AU-rich element ARE),FUNCTION: Multifunctional ATP-dependent helicase that unwinds G-quadruplex (G4) structures (PubMed:25611385). Plays a role in many biological processes such as genomic integrity, gene expression regulations and as a sensor to initiate antiviral responses (PubMed:21703541, PubMed:21590736). G4 structures correspond to helical structures containing guanine tetrads (By similarity). Binds with high affinity to and unwinds G4 structures that are formed in nucleic acids (G4-ADN and G4-RNA) (By similarity). Plays a role in genomic integrity (By similarity). Converts the G4-RNA structure present in telomerase RNA template component (TREC) into a double-stranded RNA to promote P1 helix formation that acts as a template boundary ensuring accurate reverse transcription (By similarity). Plays a role in transcriptional regulation. Resolves G4-DNA structures in promoters of genes, such as YY1, KIT/c-kit and ALPL and positively regulates their expression (PubMed:25611385) (By similarity). Plays a role in post-transcriptional regulation (By similarity). Unwinds a G4-RNA structure located in the 3'-UTR polyadenylation site of the pre-mRNA TP53 and stimulates TP53 pre-mRNA 3'-end processing in response to ultraviolet (UV)-induced DNA damage (By similarity). Binds to the precursor-microRNA-134 (pre-miR-134) terminal loop and regulates its transport into the synapto-dendritic compartment (By similarity). Involved in the pre-miR-134-dependent inhibition of target gene expression and the control of dendritic spine size (By similarity). Plays a role in the regulation of cytoplasmic mRNA translation and mRNA stability (By similarity). Binds to both G4-RNA structures and alternative non-quadruplex-forming sequence within the 3'-UTR of the PITX1 mRNA regulating negatively PITX1 protein expression (By similarity). Binds to both G4-RNA structure in the 5'-UTR and AU-rich elements (AREs) localized in the 3'-UTR of NKX2-5 mRNA to either stimulate protein translation or induce mRNA decay in an ELAVL1-dependent manner, respectively (By similarity). Binds also to ARE sequences present in several mRNAs mediating exosome-mediated 3'-5' mRNA degradation (By similarity). Involved in cytoplasmic urokinase-type plasminogen activator (uPA) mRNA decay (By similarity). Component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double-stranded RNA (dsRNA) and plays a role in the activation of a cascade of antiviral responses including the induction of pro-inflammatory cytokines via the adapter molecule TICAM1 (PubMed:21703541). Required for the early embryonic development and hematopoiesis (PubMed:22422825). Involved in the regulation of cardioblast differentiation and proliferation during heart development (PubMed:26489465). Involved in spermatogonia differentiation (PubMed:25611385). May play a role in ossification (PubMed:21590736). {ECO:0000250|UniProtKB:D4A2Z8, ECO:0000250|UniProtKB:Q05B79, ECO:0000250|UniProtKB:Q9H2U1, ECO:0000269|PubMed:21590736, ECO:0000269|PubMed:21703541, ECO:0000269|PubMed:22422825, ECO:0000269|PubMed:25611385, ECO:0000269|PubMed:26489465}.

Poids moléculaire: 113.9 kDa

UniProt: Q8VHK9

Pathways: Toll-Like Receptors Cascades