Absent in Melanoma 2 (AIM2) (AA 1-354) protein (His tag)

Détails pour le produit réf. ABIN3136782, Fournisseur: Connectez-vous pour afficher
Nom de la protéine
  • AIM2
  • PYHIN4
  • Gm1313
  • Ifi210
  • absent in melanoma 2
  • AIM2
  • Aim2
Attributs du protein
AA 1-354
3
1
1
Origine
Souris
5
1
Source
Cellules d'insectes
3
1
1
Type de proteíne
Recombinant
Purification/Conjugué
Cette AIM2 protéine est marqué à la His tag.
Application
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
Options
Fournisseur
Connectez-vous pour afficher
Supplier Product No.
Connectez-vous pour afficher
Séquence MESEYREMLL LTGLDHITEE ELKRFKYFAL TEFQIARSTL DVADRTELAD HLIQSAGAAS AVTKAINIFQ KLNYMHIANA LEEKKKEAER KLMTNTKKRG TQKVENRSQA ENCSAASATR SDNDFKEQAA TEVCPQAKPQ KKQMVAEQEA IREDLQKDPL VVTVLKAINP FECETQEGRQ EIFHATVATE TDFFFVKVLN AQFKDKFIPK RTIKISNYLW HSNFMEVTSS SVVVDVESNH EVPNNVVKRA RETPRISKLK IQPCGTIVNG LFKVQKITEE KDRVLYGIHD KTGTMEVLVL GNPSKTKCEE GDKIRLTFFE VSKNGVKIQL KSGPCSFFKV IKAAKPKTDM KSVE
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Attributs du produit
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Mouse Aim2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

Purification Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
  1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
  2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Pureté >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Stérilité 0.22 μm filtered
niveau d'endotoxine Protein is endotoxin free.
Classe de qualité Crystallography grade
Sujet Involved in innate immune response by recognizing cytosolic double-stranded DNA and inducing caspase-1-activating inflammasome formation in macrophages. Upon binding to DNA is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Detects cytosolic dsDNA of viral and bacterial origin in a non-sequence-specific manner. Can also trigger PYCARD-dependent, caspase-1-independent cell death that involves caspase-8. {ECO:0000269|PubMed:19131592, ECO:0000269|PubMed:19158675, ECO:0000269|PubMed:19158676, ECO:0000269|PubMed:20351692, ECO:0000269|PubMed:20351693, ECO:0000269|PubMed:20417169, ECO:0000269|PubMed:20457908, ECO:0000269|PubMed:21902795, ECO:0000269|PubMed:22555457, ECO:0000269|PubMed:23567559}.
Poids moléculaire 41.1 kDa Including tag.
UniProt Q91VJ1
Pathways Activation of Innate immune Response, Positive Regulation of Endopeptidase Activity
Indications d'application In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
Commentaires

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions For Research Use only
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Conseil sur la manipulation Avoid repeated freeze-thaw cycles.
Stock -80 °C
Stockage commentaire Store at -80°C.
Date de péremption Unlimited (if stored properly)
Supplier Images
 image for Absent in Melanoma 2 (AIM2) (AA 1-354) protein (His tag) (ABIN3136782) „Crystallography Grade“ protein due to multi-step, protein-specific purification process
Quantitative real-time PCR (qPCR) image for Absent in Melanoma 2 (AIM2) (AA 1-354) protein (His tag) (ABIN3136782) FAS dissolution curves
Quantitative real-time PCR (qPCR) image for Absent in Melanoma 2 (AIM2) (AA 1-354) protein (His tag) (ABIN3136782) FAS amplification curves
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