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POT1 Protein (AA 1-640) (His tag)

Crystallography grade POT1 Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
N° du produit ABIN3136797
  • Antigène Voir toutes POT1 Protéines
    POT1 (Protection of Telomeres 1 (POT1))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-640
    Origine
    Souris
    Source
    • 1
    • 1
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette POT1 protéine est marqué à la His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Séquence
    MSLVSTAPYT YTPLNLLKEG TIANVYGVVK FFKPPYVSKG TDYCSVVTIV DQTNVKLTCM LFSGNYEALP IIYKVGDIVR FHRLKIQVYK NELQGINCSG FASLTFEGTV GMPVTARTSS KVFSFTPQDQ KMVEALRVWA SKHISASSTL VQLCDAQPMQ YYDLTCQLLG KAQVDSTAFL LKVWDGTQTV LPSWRVSTQD LTFEGDLSHI ERLQSLVVDI LVYDNHVQVA RSIEVGCFLR LYSLHTKLQP GNSETSSSES LRLEFHLHGG TSYGRGIRVL PDTSPCVDQL KKALEGANLP VTETSTGICQ SENGDSSALS NSGSGAVSPY EEERCQQVSA TILTNHQHLE KTPLCAILTQ KAPQQYRVRA KLRSYLPRRL SQSVKLLCPK CHSVQEVPHG DSLDKILQDA ATEAPDIKLK ATSLYYSKVW TTEDQGGRQV AVHFVKNNGI LPASSECLIL IEGGRLCEVS KLSSKFHSVM PVRSGPESLE LLTLSAPFLI QGKVHHYGCK QCSSLKPIQN LNSRFHKGPW TPSSVAEALG VVPLQYVFVM VFTLDDGTGV LEAYLKDSEH FFKIPASEVL TDDDLQRSLE TIMDMICPPG IKVDAYPWLE CLLKSYNVTI GTERRICYQI FDTTVAENVV
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Pot1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product POT1 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    POT1 (Protection of Telomeres 1 (POT1))
    Autre désignation
    Pot1 (POT1 Produits)
    Synonymes
    HPOT1 Protein, POT1 Protein, DKFZp469P1825 Protein, pot1 Protein, 1500031H18Rik Protein, AI851169 Protein, Pot1 Protein, Pot1a Protein, protection of telomeres 1 Protein, protection of telomeres 1 L homeolog Protein, protection of telomeres 1A Protein, POT1 Protein, pot1.L Protein, Pot1a Protein, Pot1 Protein
    Sujet
    Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends, without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'-TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity (By similarity). {ECO:0000250}.
    Poids moléculaire
    71.8 kDa Including tag.
    UniProt
    Q91WC1
    Pathways
    Cycle Cellulaire, Telomere Maintenance
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