POT1 Protein (AA 1-640) (Strep Tag)

N° du produit ABIN3136797

Détail du produit

Antigène: POT1 (Protection of Telomeres 1 (POT1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-640

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette POT1 protéine est marqué à la Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Séquence: MSLVSTAPYT YTPLNLLKEG TIANVYGVVK FFKPPYVSKG TDYCSVVTIV DQTNVKLTCM LFSGNYEALP IIYKVGDIVR FHRLKIQVYK NELQGINCSG FASLTFEGTV GMPVTARTSS KVFSFTPQDQ KMVEALRVWA SKHISASSTL VQLCDAQPMQ YYDLTCQLLG KAQVDSTAFL LKVWDGTQTV LPSWRVSTQD LTFEGDLSHI ERLQSLVVDI LVYDNHVQVA RSIEVGCFLR LYSLHTKLQP GNSETSSSES LRLEFHLHGG TSYGRGIRVL PDTSPCVDQL KKALEGANLP VTETSTGICQ SENGDSSALS NSGSGAVSPY EEERCQQVSA TILTNHQHLE KTPLCAILTQ KAPQQYRVRA KLRSYLPRRL SQSVKLLCPK CHSVQEVPHG DSLDKILQDA ATEAPDIKLK ATSLYYSKVW TTEDQGGRQV AVHFVKNNGI LPASSECLIL IEGGRLCEVS KLSSKFHSVM PVRSGPESLE LLTLSAPFLI QGKVHHYGCK QCSSLKPIQN LNSRFHKGPW TPSSVAEALG VVPLQYVFVM VFTLDDGTGV LEAYLKDSEH FFKIPASEVL TDDDLQRSLE TIMDMICPPG IKVDAYPWLE CLLKSYNVTI GTERRICYQI FDTTVAENVV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: POT1 (Protection of Telomeres 1 (POT1))

Autre désignation: Pot1 (POT1 Produits)

Synonymes: HPOT1 Protein, POT1 Protein, DKFZp469P1825 Protein, pot1 Protein, 1500031H18Rik Protein, AI851169 Protein, Pot1 Protein, Pot1a Protein, protection of telomeres 1 Protein, protection of telomeres 1 L homeolog Protein, protection of telomeres 1A Protein, POT1 Protein, pot1.L Protein, Pot1a Protein, Pot1 Protein

Sujet: Protection of telomeres protein 1 (mPot1) (POT1-like telomere end-binding protein),FUNCTION: Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends, without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'-TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity (By similarity). {ECO:0000250}.

Poids moléculaire: 70.9 kDa

UniProt: Q91WC1

Pathways: Cycle Cellulaire, Telomere Maintenance