DTNBP1 Protein (AA 1-352) (Strep Tag)

N° du produit ABIN3136807

Détail du produit

Antigène: DTNBP1 (Dystrobrevin Binding Protein 1 (DTNBP1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-352

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette DTNBP1 protéine est marqué à la Strep Tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA

Séquence: MLETLRERLL SVQQDFTSGL KTLSDKSREA KVKGKPRTAP RLPKYSAGLE LLSRYEDAWA ALHRRAKECA DAGELVDSEV VMLSAHWEKK RTSLNELQGQ LQQLPALLQD LESLMASLAH LETSFEEVEN HLLHLEDLCG QCELERHKQA QAQHLESYKK SKRKELEAFK AELDTEHTQK ALEMEHTQQL KLKERQKFFE EAFQQDMEQY LSTGYLQIAE RREPMGSMSS MEVNVDVLEQ MDLMDISDQE ALDVFLNSGG EDNIVMSPGV EMESNPNQNE MSLQIPSPSE SASQPPASPS ACTDLDTADA PLIQSDEEEV QVDTALVTLH TDRKSTPGVS DDSDQCDSTQ DI
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: DTNBP1 (Dystrobrevin Binding Protein 1 (DTNBP1))

Autre désignation: Dtnbp1 (DTNBP1 Produits)

Synonymes: 5430437B18Rik Protein, AW048963 Protein, Bloc1s8 Protein, dysbindin Protein, sdy Protein, BLOC1S8 Protein, DBND Protein, HPS7 Protein, My031 Protein, SDY Protein, dtnbp1 Protein, zgc:56462 Protein, MGC83903 Protein, dbnd Protein, hps7 Protein, my031 Protein, MGC85477 Protein, DTNBP1 Protein, CG6856 Protein, Ddysb Protein, Dmel\\CG6856 Protein, si:ch211-285p6.1 Protein, dystrobrevin binding protein 1 Protein, Dysbindin Protein, dystrobrevin binding protein 1a Protein, dystrobrevin binding protein 1 L homeolog Protein, dystrobrevin binding protein 1 S homeolog Protein, dystrobrevin binding protein 1b Protein, Dtnbp1 Protein, DTNBP1 Protein, dtbp1 Protein, dtnbp1a Protein, dtnbp1.L Protein, dtnbp1.S Protein, dtnbp1 Protein, Dysb Protein, dtnbp1b Protein

Sujet: Dysbindin (Biogenesis of lysosome-related organelles complex 1 subunit 8) (BLOC-1 subunit 8) (Dysbindin-1) (Dystrobrevin-binding protein 1) (Hermansky-Pudlak syndrome 7 protein homolog) (HPS7 protein homolog),FUNCTION: Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. Associates with the BLOC-2 complex to facilitate the transport of TYRP1 independent of AP-3 function. Plays a role in synaptic vesicle trafficking and in neurotransmitter release. Plays a role in the regulation of cell surface exposure of DRD2. May play a role in actin cytoskeleton reorganization and neurite outgrowth. May modulate MAPK8 phosphorylation. Appears to promote neuronal transmission and viability through regulating the expression of SNAP25 and SYN1, modulating PI3-kinase-Akt signaling and influencing glutamatergic release. Regulates the expression of SYN1 through binding to its promoter. Modulates prefrontal cortical activity via the dopamine/D2 pathway. {ECO:0000269|PubMed:12923531, ECO:0000269|PubMed:15345706, ECO:0000269|PubMed:16448387, ECO:0000269|PubMed:16760431, ECO:0000269|PubMed:16837549, ECO:0000269|PubMed:18504299, ECO:0000269|PubMed:18555792, ECO:0000269|PubMed:18984010, ECO:0000269|PubMed:19094965, ECO:0000269|PubMed:19428785, ECO:0000269|PubMed:19546860, ECO:0000269|PubMed:19887632, ECO:0000269|PubMed:20045719, ECO:0000269|PubMed:20921223, ECO:0000269|PubMed:20956979, ECO:0000269|PubMed:21998198}.

Poids moléculaire: 39.7 kDa

UniProt: Q91WZ8

Pathways: Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling