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TRPM2 Protein (AA 1-750) (His tag)

Crystallography grade TRPM2 Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
N° du produit ABIN3136828
  • Antigène Voir toutes TRPM2 Protéines
    TRPM2 (Transient Receptor Potential Cation Channel, Subfamily M, Member 2 (TRPM2))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-750
    Origine
    • 3
    • 2
    Souris
    Source
    • 4
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette TRPM2 protéine est marqué à la His tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Séquence
    MESLDRRRTG SEQEEGFGVQ SRRATDLGMV PNLRRSNSSL CKSRRFLCSF SSEKQENLSS WIPENIKKKE CVYFVESSKL SDAGKVVCAC GYTHEQHLEV AIKPHTFQGK EWDPKKHVQE MPTDAFGDIV FTDLSQKVGK YVRVSQDTPS SVIYQLMTQH WGLDVPNLLI SVTGGAKNFN MKLRLKSIFR RGLVKVAQTT GAWIITGGSH TGVMKQVGEA VRDFSLSSSC KEGEVITIGV ATWGTIHNRE GLIHPMGGFP AEYMLDEEGQ GNLTCLDSNH SHFILVDDGT HGQYGVEIPL RTKLEKFISE QTKERGGVAI KIPIVCVVLE GGPGTLHTIY NAINNGTPCV IVEGSGRVAD VIAQVATLPV SEITISLIQQ KLSIFFQEMF ETFTENQIVE WTKKIQDIVR RRQLLTIFRE GKDGQQDVDV AILQALLKAS RSQDHFGHEN WDHQLKLAVA WNRVDIARSE IFTDEWQWKP ADLHPMMTAA LISNKPEFVR LFLENGVRLK EFVTWDTLLC LYENLEPSCL FHSKLQKVLA EEQRLAYASA TPRLHMHHVA QVLRELLGDS TQLLYPRPRY TDRPRLSMTV PHIKLNVQGV SLRSLYKRST GHVTFTIDPV RDLLIWAVIQ NHRELAGIIW AQSQDCTAAA LACSKILKEL SKEEEDTDSS EEMLALADEF EHRAIGVFTE CYRKDEERAQ KLLVRVSEAW GKTTCLQLAL EAKDMKFVSH GGIQAFLTKV WWGQLCVDNG
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Trpm2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product TRPM2 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    TRPM2 (Transient Receptor Potential Cation Channel, Subfamily M, Member 2 (TRPM2))
    Autre désignation
    Trpm2 (TRPM2 Produits)
    Synonymes
    EREG1 Protein, KNP3 Protein, LTRPC2 Protein, NUDT9H Protein, NUDT9L1 Protein, TRPC7 Protein, Trpm2-predicted Protein, TRPM2 Protein, si:ch73-263b18.1 Protein, 9830168K16Rik Protein, C79133 Protein, Trp7 Protein, Trrp7 Protein, transient receptor potential cation channel subfamily M member 2 Protein, transient receptor potential cation channel, subfamily M, member 2 Protein, transient receptor potential cation channel subfamily C member 7 Protein, TRPM2 Protein, Trpm2 Protein, trpm2 Protein, TRPC7 Protein
    Sujet
    Nonselective, voltage-independent cation channel mediating sodium and calcium ion influx in response to oxidative stress. Extracellular calcium passes through the channel and acts from the intracellular side as a positive regulator in channel activation. Activated by ADP-ribose, nicotinamide adenine dinucleotide (NAD(+)), reactive nitrogen species and arachidonic acid. Inactivated by intracellular ATP. Confers susceptibility to cell death following oxidative stress. Has ADP-ribose pyrophosphatase activity (By similarity). {ECO:0000250, ECO:0000269|PubMed:11804595}.
    Poids moléculaire
    85.6 kDa Including tag.
    UniProt
    Q91YD4
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