Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

DACH2 Protein (AA 1-634) (His tag)

Crystallography grade DACH2 Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
N° du produit ABIN3136913
  • Antigène Voir toutes DACH2 Protéines
    DACH2 (Dachshund 2 (DACH2))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-634
    Origine
    Souris
    Source
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette DACH2 protéine est marqué à la His tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Séquence
    MAVSAPPVIS ATSSSAGVPG GLFRAEPLYS SPGEPPRLTP NMINSFMANN HNGSVLGGGI GGGSGGSSNT NTNECRMVDM HGVKVASFLM DGQELICLPQ VFDLFLKHLV GGLHTVYTKL KRLDISPVVC TVEQVRILRG LGAIQPGVNR CKLITRKDFE TLFTDCTNAR RKRQMTRKQA VNSSRPGRPP KRSLGVLQDN ARLLPHAVPG LLSPGLITPT GITAAAMAEA MKLQKMKLMA MNTLQGNGSQ NGTESEPDDL NSTTGGSESS WDKDKIQSPL AASGPQHGIA HAALAGQPGL GGAPTLNPLQ QNHLLSNRLD LPFMMMPHPL LPVSLPPASV AMAMNQMNHL NTIANMAAAA QIHSPLSRAG ASVIKERIPE SPSPAPSLEE SHRPGSQTSS HPSSSVSSSP SQMDHHSERM VMMPNNREEL IVDQDNGQSI KKFQRDNKEE VPAQIPVMKS PLDKIQLAPG QALHPGFPGP FIFADSLSSV ETLLTNIQGL LKVALDNARI QEKQIQQEKK ELRIELFRER EIRENLERQL AVELQSRSTM QKRLKKEKKA KRKLQEALEF ESKRREQVEQ ALKQATSGDS GLRMLKDSGI PDIEIENSGT PHDSAAMQGG NYYCLAMAQQ LCSA
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Dach2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product DACH2 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    DACH2 (Dachshund 2 (DACH2))
    Autre désignation
    Dach2 (DACH2 Produits)
    Synonymes
    9430028N04Rik Protein, RGD1561687 Protein, dachshund family transcription factor 2 Protein, dachshund 2 (Drosophila) Protein, dachshund family transcription factor 2 L homeolog Protein, dachshund homolog 2 Protein, dachshund homolog 2 (Drosophila) Protein, DACH2 Protein, Dach2 Protein, dach2.L Protein, LOC612847 Protein, dach2 Protein
    Sujet
    Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator for SIX1 and SIX6. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. Is recruited with SIX6 to the p27Kip1 promoter in embryonal retina. SIX6 corepression seems also to involve NCOR1, TBL1, HDAC1 and HDAC3. May be involved together with PAX3, SIX1, and EYA2 in regulation of myogenesis. In the developing somite, expression of DACH2 and PAX3 is regulated by the overlying ectoderm, and DACH2 and PAX3 positively regulate each other's expression. Probably binds to DNA via its DACHbox-N domain. {ECO:0000269|PubMed:12112464, ECO:0000269|PubMed:12130660}.
    Poids moléculaire
    69.5 kDa Including tag.
    UniProt
    Q925Q8
Vous êtes ici:
Support technique