EDEM1 Protein (AA 26-652) (His tag)

N° du produit ABIN3136916

Détail du produit

Antigène: EDEM1 (ER Degradation Enhancer, Mannosidase alpha-Like 1 (EDEM1))

Type de proteíne: Recombinant

Attributs du protein: AA 26-652

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette EDEM1 protéine est marqué à la His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Séquence: GLGPSMGFYQ RFPLSFGFQR LRDPDGSGPV GPPGGPAWLH RPRRGTEGRL ETPPEPGPTP GPGVCGPAHW GYALGGGGCG PDEYERRYSG AFPPQLRAQM RDLARGMFVF GYDNYMAHAF PQDELNPIYC RGRGPDRGDP SNLNINDVLG NYSLTLVDAL DTLAIMGNSS EFQKAVKLVI NTVSFDKDST VQVFEATIRV LGSLLSAHRI ITDSKQPFGD MTIEDYDNEL LYMAHDLAVR LLPAFENTKT GIPYPRVNLK TGVPPDSNNE TCTAGAGSLL VEFGILSRLL GDSTFEWVAR RAVKALWNLR SNDTGLLGNV VNIQTGHWVG KQSGLGAGLD SFYEYLLKSY ILFGEKEDLE MFNAAYQSIQ SYLRRGREAC NEGEGDPPLY VNVNMFSGQL MNTWIDSLQA FFPGLQVLIG DVEDAICLHA FYYAIWKRYG ALPERYNWQL QAPDVLFYPL RPELVESTYL LYQATKNPFY LHVGMDILQS LEKYTKVKCG YATLHHVIDK SKEDRMESFF LSETCKYLYL LFDEENPVHK SGTRYMFTTE GHIISVDKRL RELPWKEFFS EDGERDQEEK FVHRPKSQEL RVINSSSNCN RVPDERRYSL PLKSIYMRQI DQMVGLI
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Edem1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: EDEM1 (ER Degradation Enhancer, Mannosidase alpha-Like 1 (EDEM1))

Autre désignation: Edem1 (EDEM1 Produits)

Synonymes: zgc:55816 Protein, edem Protein, EDEM1 Protein, EDEM Protein, A130059K23Rik Protein, mKIAA0212 Protein, RGD1563633 Protein, ER degradation enhancer, mannosidase alpha-like 1 Protein, ER degradation enhancing alpha-mannosidase like protein 1 Protein, ER degradation-enhancing alpha-mannosidase-like protein 1 Protein, ER degradation enhancing alpha-mannosidase like protein 1 L homeolog Protein, edem1 Protein, EDEM1 Protein, LOC100637820 Protein, edem1.L Protein, Edem1 Protein

Sujet: Extracts misfolded glycoproteins, but not glycoproteins undergoing productive folding, from the calnexin cycle. It is directly involved in endoplasmic reticulum-associated degradation (ERAD) and targets misfolded glycoproteins for degradation in an N-glycan-independent manner, probably by forming a complex with SEL1L. It has low mannosidase activity, catalyzing mannose trimming from Man8GlcNAc2 to Man7GlcNAc2. {ECO:0000250|UniProtKB:Q92611, ECO:0000269|PubMed:11375934}.

Poids moléculaire: 71.8 kDa Including tag.

UniProt: Q925U4

Pathways: ER-Nucleus Signaling