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STOML2 Protein (AA 29-353) (His tag)

Crystallography grade STOML2 Origine: Souris Hôte: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, ELISA, SDS, Crys
N° du produit ABIN3136925
  • Antigène Voir toutes STOML2 Protéines
    STOML2 (Stomatin (EPB72)-Like 2 (STOML2))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 29-353
    Origine
    • 3
    • 1
    • 1
    Souris
    Source
    • 2
    • 2
    • 1
    Escherichia coli (E. coli)
    Purification/Conjugué
    Cette STOML2 protéine est marqué à la His tag.
    Application
    Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)
    Séquence
    SSGLPRNTVI LFVPQQEAWV VERMGRFHRI LEPGLNVLIP VLDRIRYVQS LKEIVINVPE QSAVTLDNVT LQIDGVLYLR IMDPYKASYG VEDPEYAVTQ LAQTTMRSEL GKLSLDKVFR ERESLNANIV DAINQAADCW GIRCLRYEIK DIHVPPRVKE SMQMQVEAER RKRATVLESE GTRESAINVA EGKKQAQILA SEAEKAEQIN QAAGEASAVL AKAKAKAEAI RILAGALTQH NGDAAASLTV AEQYVSAFSK LAKDSNTVLL PSNPSDVTSM VAQAMGVYGA LTKAPVPGAQ NSSQSRRDVQ ATDTSIEELG RVKLS
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Stoml2 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in bacterial culture:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Endotoxin has not been removed. Please contact us if you require endotoxin removal.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product STOML2 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    STOML2 (Stomatin (EPB72)-Like 2 (STOML2))
    Autre désignation
    Stoml2 (STOML2 Produits)
    Synonymes
    zgc:63505 Protein, MGC116533 Protein, 0610038F01Rik Protein, MSLP2 Protein, SLP-2 Protein, stomatin (EPB72)-like 2 Protein, stomatin like 2 Protein, stomatin like 2 L homeolog Protein, stomatin (Epb7.2)-like 2 Protein, stoml2 Protein, STOML2 Protein, stoml2.L Protein, Stoml2 Protein
    Sujet
    Mitochondrial protein that probably regulates the biogenesis and the activity of mitochondria. Stimulates cardiolipin biosynthesis, binds cardiolipin-enriched membranes where it recruits and stabilizes some proteins including prohibitin and may therefore act in the organization of functional microdomains in mitochondrial membranes. Through regulation of the mitochondrial function may play a role into several biological processes including cell migration, cell proliferation, T-cell activation, calcium homeostasis and cellular response to stress. May play a role in calcium homeostasis through negative regulation of calcium efflux from mitochondria. Required for mitochondrial hyperfusion a pro-survival cellular response to stress which results in increased ATP production by mitochondria. May also regulate the organization of functional domains at the plasma membrane and play a role in T-cell activation through association with the T-cell receptor signaling complex and its regulation. {ECO:0000269|PubMed:19360003, ECO:0000269|PubMed:23028053}.
    Poids moléculaire
    36.5 kDa Including tag.
    UniProt
    Q99JB2
    Pathways
    SARS-CoV-2 Protein Interactome, Phosphorylation & l'infection par le SRAS-CoV-2
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