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RASSF1 Protein (AA 2-340) (His tag)

Crystallography grade RASSF1 Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
N° du produit ABIN3136966
  • Antigène Voir toutes RASSF1 Protéines
    RASSF1 (Ras Association (RalGDS/AF-6) Domain Family Member 1 (RASSF1))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 2-340
    Origine
    Souris
    Source
    • 2
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette RASSF1 protéine est marqué à la His tag.
    Application
    SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
    Séquence
    SAEPELIELR ELAPSGRIGP GRTRLERANA LRIAPGTTRN PSQQHVPGRG HRFQPAGPTT HTWCDLCGDF IWGVVRKGLQ CAHCKFTCHY RCRALVCLDC CGPRDLGWDS ALERDTNVDE AVERETPDLS QAETEQKIKD YNGQINSNLF MSLNKDGSYT GFIKVQLKLV RPVSVPSSKK PPSLQDARRG TGRSTAVKRR TSFYLPKDAI KHLHVLSRTR AREVIEALLR KFMVVDDPRK FALFERTERH GQVYLRKLSD DEQPLKLRLL AGPSEKALSF VLKENDSGEV NWDAFSMPEL HNFLRILQRE EEEHLRQILQ KYSRCRQKIQ EALHACPLG
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Rassf1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product RASSF1 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    RASSF1 (Ras Association (RalGDS/AF-6) Domain Family Member 1 (RASSF1))
    Autre désignation
    Rassf1 (RASSF1 Produits)
    Synonymes
    RASSF1 Protein, zgc:92505 Protein, wu:fc17f02 Protein, zgc:112224 Protein, 123F2 Protein, NORE2A Protein, RASSF1A Protein, RDA32 Protein, REH3P21 Protein, AA536941 Protein, AU044980 Protein, Rassf1A Protein, Rassf1B Protein, Rassf1C Protein, NPR2-like, GATOR1 complex subunit Protein, Ras association (RalGDS/AF-6) domain family 1 Protein, Ras association domain family member 1 Protein, putative rassf1 Protein, Ras association domain family member 1 L homeolog Protein, Ras association (RalGDS/AF-6) domain family member 1 Protein, NPRL2 Protein, rassf1 Protein, RASSF1 Protein, Smp_157340 Protein, rassf1.L Protein, Rassf1 Protein
    Sujet
    Potential tumor suppressor. Required for death receptor-dependent apoptosis. Mediates activation of Mediates activation of STK3/MST2 and STK4/MST1 during Fas-induced apoptosis by preventing their dephosphorylation. When associated with MOAP1, promotes BAX conformational change and translocation to mitochondrial membranes in response to TNF and TNFSF10 stimulation. Isoform A interacts with CDC20, an activator of the anaphase-promoting complex, APC, resulting in the inhibition of APC activity and mitotic progression. Inhibits proliferation by negatively regulating cell cycle progression at the level of G1/S-phase transition by regulating accumulation of cyclin D1 protein. Isoform C has been shown not to perform these roles, no function has been identified for this isoform. Isoform A disrupts interactions among MDM2, DAXX and USP7, thus contributing to the efficient activation of TP53 by promoting MDM2 self-ubiquitination in cell-cycle checkpoint control in response to DNA damage (By similarity). {ECO:0000250}.
    Poids moléculaire
    39.6 kDa Including tag.
    UniProt
    Q99MK9
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