RRBP1 Protein (AA 29-1605) (His tag)

N° du produit ABIN3137004

Détail du produit

Antigène: RRBP1 (Ribosome Binding Protein 1 (RRBP1))

Type de proteíne: Recombinant

Attributs du protein: AA 29-1605

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette RRBP1 protéine est marqué à la His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: STFSMKETSY EEALANQRKE MAKTHHQKGE KKKKEKTVEK KGKTKKKEEK PNGKIPEHDL DPNVTIILKE PVRVSAVAVA PTSVHSSVGH TPIATVPAMP QEKLASSPKD RKKKEKKVAK VEPAVSSIVN SIQVLASKSA ILEATPKEVP MVAVPPVGSK ASSPATSSQG KKGQGAQNQA KKGEGAQNQG KKGEGAQNQA KKGEGAQNQA KKGEGAQNQG KKGEGAQNQA KKGEGGQNQA KKGEGAQNQG KKGEGAQNQG KKGEGAQNQA KKGEGAQNQA KKGEGAQNQG KKGEGAQNQS KKGEGAQNQA KKGEGGQNQA KKGEGAQNQA KKGEGAQNQA KKGEGVQNQA KKGVEGAQNQ GKKGEANQNQ AKKGEGGQNQ TKKGEGPQNQ GKKGEAAQKQ DKKIEGAQNQ GKKPEGTSNQ GKKGEGAQNQ GKKGEGAQNQ SKKGEGAQNQ AKKGEGGQNQ AKKGEGAQNQ AKKGEGAQNQ AKKGEGVQNQ AKKGVEGAQN QGKKGEANQN QAKKGEGGQN QTKKGEGPQN QGKKGEAAQK QDKKIEGAQN QGKKPEGTSN QGKKGEGAQN QGKKGEGAQN QGKKGEGAQN QGKKGEGAQN QGKKGEGAQN QGKKGEGAQN QGKKGEGAQN QGKKGEGPQN QAKKGEGAQN QGKKGEGAQN QGKKGEGAQN QGKKAEGVQS QSKKGEGTQN QGKKGDGNPN QGKKGEGASN QNRKTDTVAN QGTKQEGVSN QVKKSEGSPN QGKKAEGAPN QGKKKDGSPS QAKKVDAAAN QGKKSEMAPA QGQKASMVQS QEAPKQDAPA KKKSGSRKKG EPGPPDCDGP LFLPYKTLVS TVGSMVFSEG EAQRLIEILS EKTGVIQDTW HKATQKGDPV AILKRQLQEK EKLLATEQED AAVAKSKLRE LNKEMASEKA KAAAGEAKVK KQLVAREQEI AAVQARMQAS YRDHVKEVQQ LQGKIRTLQE QLENGPNTQL ARLQQENSIL RDALNQATSQ VESKQNTELA KLRQELSKVN KELVEKSEAS RQEEQQRKAL EAKAATFEKQ VLQLQASHKE SEEALQKRLE EVTRELCRAQ TSHANLRADA EKAQEQQQRV AELHSKLQSS EVEVKSKCEE LSSLHGQLKE ARAENSQLTE RIRSIEALLE AGQAQDTQAS HAEANQQQTR LKELESQVSC LEKETSELKE AMEQQKGKNN DLREKNWKAM EALALAERAC EEKLRSLTQA KEESEKQLHL AEAQTKETLL ALLPGLSISA HQNYAEWLQE FKEKGSELLK KPPTLEPSMD IVLKLREAEE TQNSLQAECD QYRTILAETE GMLKDLQKSV EEEERVWKAK VGAAEEELHK SRVTVKHLED IVEKLKGELE SSDQVREHTS HLEAELEKHM AAASAECQNY AKEVAGLRQL LLESQSQLDE AKSEAQKQSD ELALVRQQLS DMRSHVEDGD VAGSPAVPPA EQDPMKLKTQ LERTEATLEA EQTRRQKLTA EFEEAQRTAC RIQEELEKLR AAGPLESSGK EEITQLKERL EKEKRLTSDL GRAAIKLQEL LKTTQEQLTK EKDTVKKLQE QLGKAEDGSS SKEGTSV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Rrbp1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: RRBP1 (Ribosome Binding Protein 1 (RRBP1))

Autre désignation: Rrbp1 (RRBP1 Produits)

Synonymes: 1700087N07Rik Protein, 5730465C04Rik Protein, ES/130 Protein, mKIAA1398 Protein, mRRp0 Protein, mRRp1.8 Protein, mRRp10 Protein, mRRp15a Protein, mRRp15b Protein, mRRp16.8 Protein, mRRp2 Protein, mRRp41 Protein, mRRp47 Protein, mRRp5.4 Protein, p180 Protein, ES130 Protein, RRp Protein, hES Protein, P180 Protein, rrbp1 Protein, sb:cb489 Protein, wu:fc47a01 Protein, ribosome binding protein 1 Protein, ribosome binding protein 1 homolog 180kDa (dog) Protein, ribosome binding protein 1b Protein, Rrbp1 Protein, RRBP1 Protein, rrbp1b Protein

Sujet: Acts as a ribosome receptor and mediates interaction between the ribosome and the endoplasmic reticulum membrane. {ECO:0000250}.

Poids moléculaire: 170.9 kDa Including tag.

UniProt: Q99PL5