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TRIM11 Protein (AA 1-467) (His tag)

Crystallography grade TRIM11 Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, SDS, WB, Crys
N° du produit ABIN3137006
  • Antigène Voir toutes TRIM11 Protéines
    TRIM11 (Tripartite Motif Containing 11 (TRIM11))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-467
    Origine
    • 4
    • 1
    • 1
    Souris
    Source
    • 2
    • 1
    • 1
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette TRIM11 protéine est marqué à la His tag.
    Application
    ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)
    Séquence
    MAAPDLSTNL QEEATCAICL DYFTDPVMTD CGHNFCRECI RRCWGQPEGP YACPECRELS AQRNLRPNRP LAKMAEMARR LHPPSPVPQG VCAAHREPLT TFCGDDLSLL CPICERSEHW THRVRPLQEA ADDLKGRLEK SLEHLRKQME DAMLFQAQAE ETCALWQKMV ESQRQNVLGE FERLRRLLAE EEQQLLQKLE EEELEVLPRL REGAARLGQQ STQLAALISE LESRCQLPAL GLLQDIKDAL CRVQDVKLQP PAVVPMELRT VCRVPGLVET LRRFRGDITL DPDTANPELV LSEDRRSVQR GEQRQALPDN PERFDPGPCV LGQERITSGR HYWEVEVGDQ TSWALGVCKE TANRKEKGEL SAGNGFWILV FLGSFYNSNE PAFSPLRDPP KRVGIFLDYE AGHLSFYSAT DGSLLFIFPE TLFSGTLRPL FSPLSSSPTP MTICRLIGVS GDTLGPQ
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Trim11 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product TRIM11 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    TRIM11 (Tripartite Motif Containing 11 (TRIM11))
    Autre désignation
    Trim11 (TRIM11 Produits)
    Synonymes
    BIA1 Protein, RNF92 Protein, TRIM11 Protein, tripartite motif containing 11 Protein, tripartite motif-containing 11 Protein, TRIM11 Protein, Trim11 Protein
    Sujet
    E3 ubiquitin-protein ligase that promotes the degradation of insoluble ubiquitinated proteins, including insoluble PAX6, poly-Gln repeat expanded HTT and poly-Ala repeat expanded ARX. Mediates PAX6 ubiquitination leading to proteasomal degradation, thereby modulating cortical neurogenesis. May also inhibit PAX6 transcriptional activity, possibly in part by preventing the binding of PAX6 to its consensus sequences. May contribute to the regulation of the intracellular level of HN (humanin) or HN-containing proteins through the proteasomal degradation pathway. Mediates MED15 ubiquitination leading to proteasomal degradation. May contribute to the innate restriction of retroviruses. Upon overexpression, reduces HIV-1 and murine leukemia virus infectivity, by suppressing viral gene expression. Antiviral activity depends on a functional E3 ubiquitin-protein ligase domain. May regulate TRIM5 turnover via the proteasome pathway, thus counteracting the TRIM5-mediated cross-species restriction of retroviral infection at early stages of the retroviral life cycle. {ECO:0000269|PubMed:12670303, ECO:0000269|PubMed:18248090, ECO:0000269|PubMed:18628401}.
    Poids moléculaire
    53.5 kDa Including tag.
    UniProt
    Q99PQ2
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