PARD3B Protein (AA 1-1203) (His tag)
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- Antigène Voir toutes PARD3B Protéines
- PARD3B (Par-3 Partitioning Defective 3 Homolog B (PARD3B))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-1203
- Origine
- Souris
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Source
- Cellules d'insectes
- Purification/Conjugué
- Cette PARD3B protéine est marqué à la His tag.
- Application
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Séquence
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MKVTVCFGRT GIVVPCKDGQ LRVRELTQQA LQRYLKTRDQ DPGYWVKIHH LEYTDGGILD PDDVLADVVE DKDKLIAVFD EQEPLQKTES PGGNPADRQS PDAFETEVAA QLAAFKPVGG EIVVTPSALK LGTPLLVRRS SDPAPGPHAD AQPSTASLSG QSLKPVVLDS TQNVENKEAM NGEQAGLLSL HRPKDELSDM TRAVEISGEG DPLGIHVVPF FSSLSGRILG LFIRGIEENS RCKQEGLFQE NECIVKINNV ELLDKTFAQA QDVFRQAMKS PSVILHVLLP QNREQYEKSV IGPLNIFGNN DGASRTKAAP PARGKPGLKA VHLTRASSPE GEEPASPQQS KSPRVPRLGR KPSSPSLSPL MGFGSKKNAK KIKIDLKKGP EGLGFTVVTR DSSIHGPGPI FVKNILPKGA AVKDGRLQSG DRILEVNGRD VTGRTQEELV AMLRSTKQGE TVSLVIARQE GSFLPRELKG EPDCYALSLE SSEQLTLEIP LNDSGSAGLG VSLKGNKSRE TGTDLGIFIK SIIHGGAAFK DGRLRMNDQL IAVNGETLLG KSNHEAMETL RRSMSMEGNI RGMIQLVILR RPERPLEELS ECGALSRPGF ENCQEALSTS RRNDSSILYP FGTYSPQDKR KDLLLPSDGW AENEVPPSPP PHPALEWGLE DFSHSSGVDS TGYFPDQHVN FRTVTPVRQP ELINLKASKS MDLVPDEGKV QSLADRRSDS PGKDFGPTLG LKKSSSLESL QTAVAEVRKN DLPFHRPRPH MVRGRGCNES FRAAIDKSYD GPEEADADGL SDKSSRSGHT ALNCESAPQG NPELDNVENK AKNIKKTKEK EKKKGKGKLK VKEKKLKEEH EDAERKMKRK GFGAMLRFGK KKDDKVGKAE QKGAQKSGHP EEEELERMKE ERERIGAKHQ ELREKQARGL VDYATAVTGP VHDMDDDEMD PNYARVNHFR EPCASANVFR SPSPLRAGPL AYPRDGRPLS PDHLEGLYAK VNKPYHPPAL ADSGRPMAGT TDRIQKLRKE YYQARREGFL LYEDENTRAR PSDHDLRWVS GKGPDGSTHN LRFEGMERQY ASLPRGGSAD PVDYLTASPR GRYNDRELPY YPGPHPVHAP RGSYPRPPDL RATDLRYPQY YPPPPAHQHK GPFRQDVPPS PPQHQRVPVY QEMGRAGPRG SSPDQYPYRN QDPRQKNPMT AAV
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Attributs du produit
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Pard3b Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Protein is endotoxin free.
- Classe de qualité
- Crystallography grade
- Top Product
- Discover our top product PARD3B Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Commentaires
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- PARD3B (Par-3 Partitioning Defective 3 Homolog B (PARD3B))
- Autre désignation
- Pard3b (PARD3B Produits)
- Synonymes
- ALS2CR19 Protein, PAR3B Protein, PAR3L Protein, PAR3LC Protein, PAR3beta Protein, Par3Lb Protein, 1810008K04Rik Protein, 2010002N16Rik Protein, 2810455B10Rik Protein, Als2cr19 Protein, RP23-20G7.1 Protein, par-3 family cell polarity regulator beta Protein, PARD3B Protein, Pard3b Protein
- Sujet
- Putative adapter protein involved in asymmetrical cell division and cell polarization processes. May play a role in the formation of epithelial tight junctions (By similarity). {ECO:0000250}.
- Poids moléculaire
- 133.7 kDa Including tag.
- UniProt
- Q9CSB4
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