MAGI3 Protein (AA 1-1476) (Strep Tag)

N° du produit ABIN3137189

Détail du produit

Antigène: MAGI3 (Membrane Associated Guanylate Kinase, WW and PDZ Domain Containing 3 (MAGI3))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1476

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette MAGI3 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MSKTLKKKKH WLSKVQECAV SWAGPPGDLG AEIRGGAERG EFPYLGRLRD EAGGGGGTCC VVSGKAPSPG DVLLEVNGTP VSGLTNRDTL AVIRHFREPI RLKTVKPGKV INKDLRHYLS LQFQKGSIDH KLQQVIRDNL YLRTIPCTTR APRDGEVPGV DYNFISVEQF KALEESGALL ESGTYDGNFY GTPKPPAEPS PFQPDPVDQV LFDNEFDTES QRKRTTSVSK MERMDSSLPE EEEDEDKEAV NGSGSMETRE MHSETSDCWM KTVPSYNQTN SSMDFRNYMM RDENLEPLPK NWEMAYTDTG MIYFIDHNTK TTTWLDPRLC KKAKAPEDCE DGELPYGWEK IEDPQYGTYY VDHLNQKTQF ENPVEEAKRK KQLGQAEIHS AKTDVERAHF TRDPSQLKGV LVRASLKKST MGFGFTIIGG DRPDEFLQVK NVLKDGPAAQ DGKIAPGDVI VDINGNCVLG HTHADVVQMF QLVPVNQYVN LTLCRGYPLP DDSEDPVVDI VAATPVINGQ SLTKGETCMN TQDFKLGAMV LDQNGKSGQI LASDRLNGPS ESSEQRASLA SSGSSQPELV TIPLIKGPKG FGFAIADSPT GQKVKMILDS QWCQGLQKGD IIKEIYHQNV QNLTHLQVVE VLKQFPVGAD VPLLILRGGP CSPTKTAKTK TDTKENSGSL ETINEPIPQP MPFPPSIIRS GSPKLDPSEV YLKSKTLYED KPPNTKDLDV FLRKQESGFG FRVLGGDGPD QSIYIGAIIP LGAAEKDGRL RAADELMCID GIPVKGKSHK QVLDLMTTAA RNGHVLLTVR RKIFYGEKQP EDESHQAFSQ NGSPRLNRAE LPTRSAPQEA YDVTLQRKEN EGFGFVILTS KSKPPPGVIP HKIGRVIDGS PADRCGGLKV GDHISAVNGQ SIVDLSHDNI VQLIKDAGVT VTLTVVAEEE HHGPPSGTNS ARQSPALQHR PMGQAQANHI PGDRIALEGE IGRDVCSSYR HSWSDHKHLA QPDTAVISVV GSRHNQSLGC YPVELERGPR GFGFSLRGGK EYNMGLFILR LAEDGPAIKD GRIHVGDQIV EINGEPTQGI THTRAIELIQ AGGNKVLLLL RPGTGLIPDH GDWDTNSPSS SNVIYDEQPP PLPSSHSAST FEESHVPATE DSLTRVQICE KAEELKDTVQ EKKSTLNGSQ PEMKYQSVHK TMSKKDPPRG SGHGEKSRLK GENGVTRRGR SASPKKSVNR HSEEHLEKIP RPLKSDPKEK SRDRSLSPRK GESKGRLTIK AGSGQDPYRK DRGRSSSPKK QQKIGGNSLS NTEGKLSEAG SRRAAGHPRD STEQLPDGRE KSGVSRKDLK QSQPGKTRTK SPEKKSSKVD ETSLPSKKTS STAGRVVSEK EKGKKPTAGE TSRETVEHTQ ISAKQLKQEA QEKTALGNAD DHKGRESEVT DRCRERAGCT PQSSSLVKKA PITPGPWRVP RANKVTGTTG MADKQL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: MAGI3 (Membrane Associated Guanylate Kinase, WW and PDZ Domain Containing 3 (MAGI3))

Autre désignation: Magi3 (MAGI3 Produits)

Synonymes: Slipr Protein, MAGI-3 Protein, RP4-730K3.1 Protein, dJ730K3.2 Protein, 4732496O19Rik Protein, 6530407C02Rik Protein, AA407180 Protein, AI120132 Protein, mKIAA1634 Protein, membrane associated guanylate kinase, WW and PDZ domain containing 3 Protein, Magi3 Protein, MAGI3 Protein

Sujet: Membrane-associated guanylate kinase, WW and PDZ domain-containing protein 3 (Membrane-associated guanylate kinase inverted 3) (MAGI-3),FUNCTION: Acts as a scaffolding protein at cell-cell junctions, thereby regulating various cellular and signaling processes. Cooperates with PTEN to modulate the kinase activity of AKT1. Its interaction with PTPRB and tyrosine phosphorylated proteins suggests that it may link receptor tyrosine phosphatase with its substrates at the plasma membrane. In polarized epithelial cells, involved in efficient trafficking of TGFA to the cell surface. Regulates the ability of LPAR2 to activate ERK and RhoA pathways. Regulates the JNK signaling cascade via its interaction with FZD4 and VANGL2. {ECO:0000269|PubMed:15195140, ECO:0000269|PubMed:15652357}.

Poids moléculaire: 161.7 kDa

UniProt: Q9EQJ9