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NCKIPSD Protein (AA 1-714) (Strep Tag)

Crystallography grade NCKIPSD Origine: Souris Hôte: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
N° du produit ABIN3137230
  • Antigène Voir toutes NCKIPSD Protéines
    NCKIPSD (NCK Interacting Protein with SH3 Domain (NCKIPSD))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-714
    Origine
    Souris
    Source
    • 2
    • 1
    • 1
    • 1
    Tobacco (Nicotiana tabacum)
    Purification/Conjugué
    Cette NCKIPSD protéine est marqué à la Strep Tag.
    Application
    ELISA, Western Blotting (WB), SDS-PAGE (SDS)
    Séquence
    MYRALYAFRS AEPNAMAFAA GETFLVLERS STHWWLAARA RSGETGYVPP AYLHRLQGME QDVLQAIDRA IEAVHNTAMR DGGKYSLEQR GVLQKLIHHR KETLSRRGTS ASSATVMTPS TSDHHLDAAV SRQPNGVCRT GFERQHSLPS SEHLGTDGAL YQVPPQPRRA APTTPPPPVK RRDREALVIS GSGGRTAIPS GGSSVSSGSS ASSTSMDTLY TGSSPSELGP SCSPTPPPVP RRGAHTTVSQ PQPSPSKAPS PEPPTEEVAA ETNSTPDDLE AQDALSPETT EEKAAAETVV PRTIGAELME LVRRNTGLSH ELCRVAIGVV VGHIQATVPA SSPIMEQVLL SLVEGKDLST ALPSGQVCHD QQRLEVIFAD LARRKDDAQQ RSWALYEDED VIRCYLEELL HILTDADPEV CKKMCKRSDF ESVLALVAYY QMEHRASLRL LLLKCFGAMC SLDAAIISTL VSSVLPVELA RDMQTDTQDH QKLCYSALVL AMVFSMGEAV PYAHYEHLGT PFAQFLLSIV EDGLPMDTTE QLPDLCMNLL LALNLHLTAP EQNVIMAALS RHTNVKIFSE KLLLLLNRGD DPVRIFRHEP QPPHSVLKFL QDVFSSSATA AIFYHTDMMA LIDITVRQIA DLSPGDKLRM EYLSLMHAVV RSTPYLQHRH RLSDLQATLR RILTEEEASP QCQMDRMIVQ EMYKEFPDLG EVPS
    Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.
    Attributs du produit
    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
    • These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    Expression System:

    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
    • We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
    1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    niveau d'endotoxine
    Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product NCKIPSD Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Commentaires

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    NCKIPSD (NCK Interacting Protein with SH3 Domain (NCKIPSD))
    Autre désignation
    Nckipsd (NCKIPSD Produits)
    Synonymes
    AF3P21 Protein, DIP Protein, DIP1 Protein, ORF1 Protein, SPIN90 Protein, VIP54 Protein, WASLBP Protein, WISH Protein, Wasbp Protein, SH3 adapter protein SPIN90 Protein, NCK interacting protein with SH3 domain Protein, CpipJ_CPIJ019280 Protein, NCKIPSD Protein, Nckipsd Protein
    Sujet
    NCK-interacting protein with SH3 domain (54 kDa VacA-interacting protein) (VIP54) (90 kDa N-WASP-interacting protein) (90 kDa SH3 protein interacting with Nck) (SH3 adapter protein SPIN90) (WASP-interacting SH3-domain protein) (WISH) (Wiskott-Aldrich syndrome protein-binding protein) (N-WASP-binding protein),FUNCTION: Has an important role in stress fiber formation induced by active diaphanous protein homolog 1 (DRF1) (By similarity). Induces microspike formation, in vivo. In vitro, stimulates N-WASP-induced ARP2/3 complex activation in the absence of CDC42. May play an important role in the maintenance of sarcomere and/or in the assembly of myofibrils into sarcomeres. Implicated in regulation of actin polymerization and cell adhesion. {ECO:0000250}.
    Poids moléculaire
    78.6 kDa
    UniProt
    Q9ESJ4
    Pathways
    Protein targeting to Nucleus
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