DKC1 Protein (AA 2-509) (His tag)

N° du produit ABIN3137237

Détail du produit

Antigène: DKC1 (Dyskeratosis Congenita 1, Dyskerin (DKC1))

Type de proteíne: Recombinant

Attributs du protein: AA 2-509

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette DKC1 protéine est marqué à la His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Séquence: ADAEVITFPK KHKKKKDRKP LQEDDVAEIQ HAEEFLIKPE SKVAQLDTSQ WPLLLKNFDK LNVRTAHYTP LPCGSNPLKR EIGDYIRTGF INLDKPSNPS SHEVVAWIRR ILRVEKTGHS GTLDPKVTGC LIVCIERATR LVKSQQSAGK EYVGIVRLHN AIEGGTQLSR ALETLTGALF QRPPLIAAVK RQLRVRTIYE SKMIEYDPER RLGIFWVSCE AGTYIRTLCV HLGLLLGVGG QMQELRRVRS GVMSEKDHMV TMHDVLDAQW LYDNHKDESY LRRVVYPLEK LLTSHKRLVM KDSAVNAICY GAKIMLPGLL RYEDGIEVNQ EIVVITTKGE AICMAIALMT TAVISTCDHG IVAKIKRVIM ERDTYPRKWG LGPKASQKKM MIKQGLLDKH GKPTDNTPAT WKQDYIDYSD SGKNTLVTEA VQAPQLAAEA VNVIKRKRDS ESESDETPTV PQLKEKKKKK DKKPKTVLES GGETGDGDND TTKKKKKKKV KVVEEMSE
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Dkc1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: DKC1 (Dyskeratosis Congenita 1, Dyskerin (DKC1))

Autre désignation: Dkc1 (DKC1 Produits)

Synonymes: CBF5 Protein, DKC Protein, DKCX Protein, NAP57 Protein, NOLA4 Protein, XAP101 Protein, dyskerin Protein, fv62a07 Protein, wu:fa28f10 Protein, wu:fc87a02 Protein, wu:fi24a05 Protein, wu:fv62a07 Protein, zgc:110395 Protein, DKC1 Protein, cbf5 Protein, dkc Protein, nap57 Protein, nola4 Protein, xap101 Protein, BC068171 Protein, Nap57 Protein, AtCBF5 Protein, AtNAP57 Protein, homologue of NAP57 Protein, dyskerin pseudouridine synthase 1 Protein, microRNA 664b Protein, dyskeratosis congenita 1, dyskerin Protein, dyskeratosis congenita 1, dyskerin L homeolog Protein, homologue of NAP57 Protein, DKC1 Protein, MIR664B Protein, dkc1 Protein, dkc1.L Protein, Dkc1 Protein, NAP57 Protein

Sujet: Required for ribosome biogenesis and telomere maintenance. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Each rRNA can contain up to 100 pseudouridine ('psi') residues, which may serve to stabilize the conformation of rRNAs. Also required for correct processing or intranuclear trafficking of TERC, the RNA component of the telomerase reverse transcriptase (TERT) holoenzyme. {ECO:0000269|PubMed:12522253, ECO:0000269|PubMed:15240872}.

Poids moléculaire: 58.2 kDa Including tag.

UniProt: Q9ESX5

Pathways: Telomere Maintenance