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ORC3 Protein (AA 1-715) (His tag)

Crystallography grade ORC3 Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
N° du produit ABIN3137304
  • Antigène Voir toutes ORC3 Protéines
    ORC3 (Origin Recognition Complex, Subunit 3 (ORC3))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-715
    Origine
    • 3
    • 1
    Souris
    Source
    • 2
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette ORC3 protéine est marqué à la His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Séquence
    MHTGPRTMAT SSVSKGCFVF KPDFKKRKVF VPIEDYFNNE ELDSEDSKLR FETYSLLWQR MKSETEQLQE ELNENLFDNL VDFLQKSHPE FQKNSRDWGS QMKFREIPTA ALILGVNVTD HDVILRSLTE TLQNNVTPYV VSLQAKDCPD VKHFLQKFTS QLMDCCVDRH SKEVTSGKAL KKTNYSMDSL CSWYSAVTQK ADHKVTIKKR TASGHWRSPP VVLILKSMES FTSKVLQDFI TISSQHLHEF PLILIFGIAT SPVIIHRLLP HSVSSLLCVE LFQSLSCEQH LTVVLDKLLL TPQFPFKLSK KALQVLTNIF LYHDFSIQSF IKGIKLSLLE HFYSQPLSVL CCDLSEAKKR VNVFSVSQCE NIRRLPSFRR YVENQPLGKQ VALLTNETFL KEKTQSLLED LHVYHINYFL VLRCLHNFTS SLPKYPLGRQ IRELYCTCLE KKIWDSEEYK SALQLLRMLA KDELVSILQR CIEVLDSSTE KQLGNTTQKI KDFLTQFQNL DADSKEEEDA CGSQPKGLQK TDLYHLQKSL LEMKELRRTK KPTKFEMLRE NVMNFIDNLV RDYLLPPESQ PLHEVVYFSA ANTLREHLNA APRIALHTAL NNPYYYLKNE ELEGCIPNTA PDICIAYKLH LECSLLINLV DWAEAFATVV TAAEKMDANS TVSEEMSEVI HARFIRAVSE LELLGFIKPT KQKTDHVARL TWGGC
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Orc3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product ORC3 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    ORC3 (Origin Recognition Complex, Subunit 3 (ORC3))
    Autre désignation
    Orc3 (ORC3 Produits)
    Synonymes
    CG4088 Protein, DmORC3 Protein, DmOrc3 Protein, Dmel\\CG4088 Protein, LAT Protein, ORC Protein, ORC3 Protein, Orc3 Protein, l(2)49Fd Protein, l(2)vr6 Protein, vr-6 Protein, vr6 Protein, ORC3L Protein, orc3 Protein, ATORC3 Protein, F5E19.30 Protein, F5E19_30 Protein, ORIGIN RECOGNITION COMPLEX SUBUNIT 3 Protein, origin recognition complex subunit 3 Protein, Afu8g04240 Protein, DDBDRAFT_0216767 Protein, DDBDRAFT_0233773 Protein, DDB_0216767 Protein, DDB_0233773 Protein, LOC100226325 Protein, LATHEO Protein, Orc3l Protein, Origin recognition complex subunit 3 Protein, origin recognition complex subunit 3 Protein, origin recognition complex, subunit 3 Protein, Orc3 Protein, ORC3 Protein, orc3 Protein, AFUA_8G04240 Protein, BDBG_01002 Protein, orcC Protein
    Sujet
    Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication (By similarity). Binds histone H3 and H4 trimethylation marks H3K9me3, H3K27me3 and H4K20me3 (By similarity). {ECO:0000250}.
    Poids moléculaire
    83.3 kDa Including tag.
    UniProt
    Q9JK30
    Pathways
    Mitotic G1-G1/S Phases, DNA Replication, Feeding Behaviour, Synthesis of DNA
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