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DACH1 Protein (AA 1-751) (His tag)

Crystallography grade DACH1 Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
N° du produit ABIN3137391
  • Antigène Voir toutes DACH1 Protéines
    DACH1 (Dachshund 1 (DACH1))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-751
    Origine
    Souris
    Source
    • 1
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette DACH1 protéine est marqué à la His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Séquence
    MAVPAALIPP TQLVPPQPPI STSASSSGTT TSTSSATSSP APSIGPPASS GPTLFRPEPI ASSASSSAAA TVTSPGGGGG GSGGGGGSGG NGGGGGSNCN PSLAAGSSGG GVSAGGGGAS STPITASTGS SSSSSSSSSS SSSSSSSSSS SSSSSSCGPL PGKPVYSTPS PVENTPQNNE CKMVDLRGAK VASFTVEGCE LICLPQAFDL FLKHLVGGLH TVYTKLKRLE ITPVVCNVEQ VRILRGLGAI QPGVNRCKLI SRKDFETLYN DCTNASSRPG RPPKRTQSVT SPENSHIMPH SVPGLMSPGI IPPTGLTAAA AAAAAATNAA IAEAMKVKKI KLEAMSNYHA SNNQHGADSE NGDMNSSVGS SGGSWDKETL HSPPSQGSQA PVAHARMPAA FSLPVSHPLN HLQHSHLPPN GLELPFMMMP HPLIPVSLPP ASVTMAMSQM NHLSTIANMA AAAQVQSPPS RVETSVIKER VPDSPSPAPS LEEGRRPGSH PSSHRSSSVS SSPARTESSS DRIPVHQNGL SMNQMLMGLS PNVLPGPKEG DLAGHDMGHE SKRIHIEKDE TPLSTPTARD SIDKLSLTGH GQPLPPGFPS PFLFPDGLSS IETLLTNIQG LLKVAIDNAR AQEKQVQLEK TELKMDFLRE RELRETLEKQ LAMEQKNRAI VQKRLKKEKK AKRKLQEALE FETKRREQAE QTLKQAASAD SLRVLNDSLT PEIEADRSGG RADAERTIQD GRLYLKTTVM Y
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Dach1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product DACH1 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    DACH1 (Dachshund 1 (DACH1))
    Autre désignation
    Dach1 (DACH1 Produits)
    Synonymes
    DACH Protein, AI182278 Protein, Dac Protein, Dach Protein, E130112M23Rik Protein, dach Protein, dachshund family transcription factor 1 Protein, dachshund 1 (Drosophila) Protein, dachshund family transcription factor 1 S homeolog Protein, dachshund homolog 1 Protein, DACH1 Protein, Dach1 Protein, dach1.S Protein, dach1 Protein, LOC100550020 Protein
    Sujet
    Transcription factor that is involved in regulation of organogenesis. Seems to be a regulator of SIX1, SIX6 and probably SIX5. Corepression of precursor cell proliferation in myoblasts by SIX1 is switched to coactivation through recruitment of EYA3 to the SIX1-DACH1 complex. Transcriptional activation seems also to involve association of CREBBP. Seems to act as a corepressor of SIX6 in regulating proliferation by directly repressing cyclin-dependent kinase inhibitors, including the p27Kip1 promoter. Inhibits TGF-beta signaling through interaction with SMAD4 and NCOR1 (By similarity). Binds to chromatin DNA via its DACHbox-N domain. {ECO:0000250, ECO:0000269|PubMed:12130660, ECO:0000269|PubMed:12215533, ECO:0000269|PubMed:14628042}.
    Poids moléculaire
    78.9 kDa Including tag.
    UniProt
    Q9QYB2
    Pathways
    Feeding Behaviour
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