HTRA1 Protein (AA 23-480) (His tag)

N° du produit ABIN3137441

Détail du produit

Antigène: HTRA1 (HtrA Serine Peptidase 1 (HTRA1))

Type de proteíne: Recombinant

Attributs du protein: AA 23-480

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette HTRA1 protéine est marqué à la His tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)

Séquence: LPSGTGRSAP AATVCPEHCD PTRCAPPPTD CEGGRVRDAC GCCEVCGALE GAACGLQEGP CGEGLQCVVP FGVPASATVR RRAQAGLCVC ASSEPVCGSD AKTYTNLCQL RAASRRSEKL RQPPVIVLQR GACGQGQEDP NSLRHKYNFI ADVVEKIAPA VVHIELYRKL PFSKREVPVA SGSGFIVSED GLIVTNAHVV TNKNRVKVEL KNGATYEAKI KDVDEKADIA LIKIDHQGKL PVLLLGRSSE LRPGEFVVAI GSPFSLQNTV TTGIVSTTQR GGKELGLRNS DMDYIQTDAI INYGNSGGPL VNLDGEVIGI NTLKVTAGIS FAIPSDKIKK FLTESHDRQA KGKAVTKKKY IGIRMMSLTS SKAKELKDRH RDFPDVLSGA YIIEVIPDTP AEAGGLKEND VIISINGQSV VTANDVSDVI KKENTLNMVV RRGNEDIVIT VIPEEIDP
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Htra1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: HTRA1 (HtrA Serine Peptidase 1 (HTRA1))

Autre désignation: Htra1 (HTRA1 Produits)

Synonymes: htra1 Protein, zgc:92029 Protein, zgc:172061 Protein, l56 Protein, htra Protein, Xhtra1 Protein, prss11 Protein, MGC145723 Protein, ARMD7 Protein, CARASIL Protein, HtrA Protein, L56 Protein, ORF480 Protein, PRSS11 Protein, AI429470 Protein, HTRA Protein, Prss11 Protein, RSPP11 Protein, HTRA1 Protein, HtrA serine peptidase 1a Protein, HtrA serine peptidase 1 Protein, HtrA serine peptidase 1b Protein, Serine protease HTRA1 Protein, HtrA serine peptidase 1 S homeolog Protein, htra1a Protein, HTRA1 Protein, htra1b Protein, htra1 Protein, Htra1 Protein, htra1.S Protein

Sujet: Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, but it is unclear whether it leads to the proteolytic degradation of TGF-beta proteins themselves (PubMed:18551132) or not (PubMed:14973287). By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets. {ECO:0000269|PubMed:14973287, ECO:0000269|PubMed:15993670, ECO:0000269|PubMed:18551132, ECO:0000269|PubMed:22049084}.

Poids moléculaire: 49.8 kDa Including tag.

UniProt: Q9R118

Pathways: Growth Factor Binding