FZR1 Protein (AA 1-493) (His tag)
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- Antigène Voir toutes FZR1 Protéines
- FZR1 (Fizzy/cell Division Cycle 20 Related 1 (FZR1))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-493
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Origine
- Souris
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Source
- Cellules d'insectes
- Purification/Conjugué
- Cette FZR1 protéine est marqué à la His tag.
- Application
- SDS-PAGE (SDS), ELISA, Western Blotting (WB), Crystallization (Crys)
- Séquence
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MDQDYERRLL RQIIIQNENT VPCVSEMRRT LTPANSPVSS PSKHGDRFIP SRAGANWSVN FHRINENEKS PSQNRKAKDA TSDNGKDGLA YSALLKNELL GAGIEKVQDP QTEDRRLQPS TPEHKGLFTY SLSSKRSSPD DGNDVSPYSL SPVSNKSQKL LRSPRKPTRK ISKIPFKVLD APELQDDFYL NLVDWSSLNV LSVGLGTCVY LWSACTSQVT RLCDLSVEGD SVTSVGWSER GNLVAVGTHK GFVQIWDAAA GKKLSMLEGH TARVGALAWN ADQLSSGSRD RMILQRDIRT PPLQSERRLQ GHRQEVCGLK WSTDHQLLAS GGNDNKLLVW NHSSLSPVQQ YTEHLAAVKA IAWSPHQHGL LASGGGTADR CIRFWNTLTG QPLQCIDTGS QVCNLAWSKH ANELVSTHGY SQNQILVWKY PSLTQVAKLT GHSYRVLYLA MSPDGEAIVT GAGDETLRFW NVFSKTRSTK ESVSVLNLFT RIR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Attributs du produit
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Fzr1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Protein is endotoxin free.
- Classe de qualité
- Crystallography grade
- Top Product
- Discover our top product FZR1 Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Commentaires
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- FZR1 (Fizzy/cell Division Cycle 20 Related 1 (FZR1))
- Autre désignation
- Fzr1 (FZR1 Produits)
- Synonymes
- CDH1-C Protein, FYR Protein, FZR Protein, CDH1 Protein, fzr1 Protein, CDC20C Protein, FZR2 Protein, HCDH Protein, HCDH1 Protein, AW108046 Protein, Cdh1 Protein, Fyr Protein, zgc:55450 Protein, fizzy and cell division cycle 20 related 1 Protein, fizzy/cell division cycle 20 related 1 Protein, fizzy-related protein homolog Protein, fizzy/cell division cycle 20 related 1 (Drosophila) Protein, fizzy/cell division cycle 20 related 1a Protein, Fzr1 Protein, CDH1-C Protein, FZR1 Protein, LOC100027495 Protein, LOC100179951 Protein, fzr1a Protein
- Sujet
- Key regulator of ligase activity of the anaphase promoting complex/cyclosome (APC/C), which confers substrate specificity upon the complex. Associates with the APC/C in late mitosis, in replacement of CDC20, and activates the APC/C during anaphase and telophase. The APC/C remains active in degrading substrates to ensure that positive regulators of the cell cycle do not accumulate prematurely. At the G1/S transition FZR1 is phosphorylated, leading to its dissociation from the APC/C. Following DNA damage, it is required for the G2 DNA damage checkpoint: its dephosphorylation and reassociation with the APC/C leads to the ubiquitination of PLK1, preventing entry into mitosis (By similarity). {ECO:0000250}.
- Poids moléculaire
- 55.6 kDa Including tag.
- UniProt
- Q9R1K5
- Pathways
- DNA Replication, Synthesis of DNA
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