NUP160 Protein (AA 1-1402) (Strep Tag)

N° du produit ABIN3137576

Détail du produit

Antigène: NUP160 (Nucleoporin 160kDa (NUP160))

Type de proteíne: Recombinant

Attributs du protein: AA 1-1402

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette NUP160 protéine est marqué à la Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Séquence: MAAAGSLERS FVELSGAERE RPRHFREFTV CDIGTASAAF GTVKYSESAG GFYYVESGKL FSITRNRFIH WKTSGDTLEL VEESLDLNLL NNAVRLKFQN YNILPGGVHV SETQNHVIIL ILTNQTVHRL ILPHPSRMYR SELVTESQMQ SIFTDIGKVD FRDPCNSQLI PSVPGLSPGS TTSAAWLSSD GEALFALPSA SGGIFVLKLP PYDVPGIASV VELKQSSVMQ RLLTGWMPTA IRGDHGPSDR ALSLAVHCVE HDAFIFALCQ DHKLRMWSYK DQMCLMVADM LEYVPVNKDL RLTAGTGHKL RLAYSPSMGL YLGIYMHAPK RGQFCVFQLV STENNRYSLD HISSLFTSQE TLVDFALTST DIWALWHDAE NQTIVKYINF EHNVAGQWNP VFMQPLPEEE IVIRDDQDPR EMYLRSLFTP GHFINAALCK ALQIFCRGTE RNLDLSWNEL KKEITLAVEN ELQGSVTEYE FSQDEFRTLQ QEFWCKFYAC VLQYQEALSH PLALHLNPVT NMVCLLKKGY LSFLVPSSLV DHLYLLPDEH LLTEDETTIS DDADVARDVL CLIKCLRMIG ESVTMDMAVL METSCYNLQS PEKAAEHILE DLITIDVENV MEDICSKLQE IRNPVHAIGL LIREMDYETE VEMEKGFDPA QPLNVRMNLS QLYGSSTAGY IVCRGVYKIA STRFLICRDL LILQQLLTRL GDAVILGAGQ LFQAQQDLLH RTAPLLLSYY LIKWASQCLA TDVPVDTLES NLQHLSVLEL TDSGALMANK LVSSPQTIME LFFQEVARKQ IISHLFSQPK APLSQTGLNW PEMITAVTGY LLQLLWPSNP GCLFLECLMG NCQYVQLQDY IQLLHPWCQV NVGSCRFMLG RCYLVTGEVQ KALECFCQAA SEVGKEEFLD RLIRSEDGEI VSTPKLQYYD KVLRLLDVVG LPELVIQLAT SAITEAGDDW KSQATLRTCI FKHHLDLGHN SQAYEALTQI PDSSRQLDCL RQLVVVLCER SQLQDLVEFP YVNLHNEVVG IIESRARAVD LMTHNYYELL YAFHIYRHNY RKAGTVMFEY GMRLGREVRT LRGLEKQGNC YLAAINCLRL IRPEYAWIVQ PASGAVSDRP GASPKRNHDG ECTAAPTNRQ IEILELEDLE KEYSLARIRL TLARHDPSVI AIAGSSSAKE MSALLVQAGL FDTAISLCQT FTLPLTPVFE GLAFKCIKLQ FGGEAAQGEA WSWLATNQLS SVITTKESSA TDEAWRLLST YLERYKVQNN LYHHCVINKL LSHGVPLPNW LINSYKKVDA AELLRLYLNY DLLEEAVDLV SEYVDAVLGK GHQYFGIEFP LSATAPMVWL PYSSIDQLLQ ALGENSANSH NIILSQKILD KLEDYQQKVD KATRDLLYRR DL
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: NUP160 (Nucleoporin 160kDa (NUP160))

Autre désignation: Nup160 (NUP160 Produits)

Synonymes: CG4738 Protein, Dmel\\CG4738 Protein, Nup160[mel] Protein, l(2)SH2 2055 Protein, l(2)SH2055 Protein, nup160 Protein, MGC78785 Protein, 160kDa Protein, 2810011M03Rik Protein, AA414952 Protein, AU020188 Protein, Gtl-13 Protein, Gtl1-13 Protein, Gtl13 Protein, mKIAA0197 Protein, nucleoporin 160 Protein, Nucleoporin 160kD Protein, nucleoporin 160kDa L homeolog Protein, nucleoporin 160kDa Protein, NUP160 Protein, Nup160 Protein, nup160.L Protein, nup160 Protein

Sujet: Nuclear pore complex protein Nup160 (160 kDa nucleoporin) (Gene trap locus 1-13 protein) (GTL-13) (Nucleoporin Nup160),FUNCTION: Functions as a component of the nuclear pore complex (NPC) (PubMed:11564755, PubMed:11684705). Involved in poly(A)+ RNA transport (PubMed:11684705). {ECO:0000269|PubMed:11564755, ECO:0000269|PubMed:11684705}.

Poids moléculaire: 158.2 kDa

UniProt: Q9Z0W3

Pathways: Protein targeting to Nucleus