Elf4 Protein (AA 1-655) (Strep Tag)

N° du produit ABIN3137641

Détail du produit

Antigène: Elf4 (E74 like ETS transcription factor 4 (Elf4))

Type de proteíne: Recombinant

Attributs du protein: AA 1-655

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette Elf4 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MAIALQPSDL VFEFASNGMD DIHQLEDPSV FPAVIVEQVP YPELVHLCSG LDLDEVHNGI IRDRTLCMTQ DQILEGSILL TDDDVSTSNN VSSTEVLFNV ATPSDVLDEK QIFSSPEVLS DSNSVQAINL PNFLLSTPEP DDLKKTSDAG DQKEHSEEEK VSREENLRKM GKARKRNRKT KNNRSTSPVT DPSMPIRKKS KDGKGSTIYL WEFLLALLQD RNTCPKYIKW TQREKGIFKL VDSKAVSKLW GKQKNKPDMN YETMGRALRY YYQRGILAKV EGQRLVYQFK EMPKDLVVID DEEESPETPE DSSQASTSST PSTSTIRRAS SRVGTRASPE DKDNPPWEKP KVQHTGLQPS ASLELGLSVD EEVPTTSTML ASPLQSQARL TKTVSSSPAP SNIHLGVAPV GPGSTVTLQT IPLTTVLTNG PPASTTAPTQ LVLQSVPQVS TFKDTFTLQT SFPLNTNLQE NQVATQGAPL ILSGLPQLLA GANPQSNPAP SQVIGAGSAG PSSQPPGTVI AAFIRTSSGT SVPVVKEGPL RSSSYVQGVV TGAPVEGLLV PEETLRELLR DQGHLQPLPS QVLSRGSHNL SLVGNQTLSP PSHPTVGLTP VAELELSSGS GPLFVTEPSV TRSPTQAPFS PFNPTSLIKM EPQDI
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: Elf4 (E74 like ETS transcription factor 4 (Elf4))

Autre désignation: Elf4 (Elf4 Produits)

Synonymes: ELF4 Protein, RGD1560743 Protein, ELFR Protein, MEF Protein, AV314029 Protein, BC042423 Protein, ENSMUSG00000053512 Protein, Gm9907 Protein, Mef Protein, E74 like ETS transcription factor 4 Protein, E74-like factor 4 (ets domain transcription factor) Protein, ELF4 Protein, Elf4 Protein

Sujet: ETS-related transcription factor Elf-4 (E74-like factor 4) (Myeloid Elf-1-like factor),FUNCTION: Transcriptional activator that binds to DNA sequences containing the consensus 5'-WGGA-3'. Transactivates promoters of the hematopoietic growth factor genes CSF2, IL3, IL8, and of LYZ. Acts synergistically with RUNX1 to transactivate the IL3 promoter (By similarity). Transactivates the PRF1 promoter in natural killer (NK) cells and in CD8+ T cells (PubMed:34326534). Plays a role in the development and function of NK and NK T-cells and in innate immunity. Controls the proliferation and homing of CD8+ T-cells via the Kruppel-like factors KLF4 and KLF2. Controls cell senescence in a p53-dependent manner. Can also promote cellular transformation through inhibition of the p16 pathway. Is a transcriptional regulator of inflammation, controlling T-helper 17 (Th17) cells and macrophage inflammatory responses. Required for sustained transcription of anti-inflammatory genes, including IL1RN (PubMed:34326534, PubMed:35266071). Is a negative regulator of pro-inflammatory cytokines expression, including IL17A, IL1B, IL6, TNFA and CXCL1 (PubMed:34326534, PubMed:35266071). Down-regulates expression of TREM1, a cell surface receptor involved in the amplification of inflammatory responses (PubMed:34326534, PubMed:35266071). {ECO:0000250, ECO:0000269|PubMed:12387738, ECO:0000269|PubMed:19380490, ECO:0000269|PubMed:19412182, ECO:0000269|PubMed:34326534, ECO:0000269|PubMed:35266071}.

Poids moléculaire: 70.8 kDa

UniProt: Q9Z2U4

Pathways: Positive Regulation of Endopeptidase Activity