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CRLF2 Protein (AA 20-359) (rho-1D4 tag)

Crystallography grade CRLF2 Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
N° du produit ABIN3126096
  • Antigène Voir toutes CRLF2 Protéines
    CRLF2 (Cytokine Receptor-Like Factor 2 (CRLF2))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 20-359
    Origine
    • 12
    • 7
    • 1
    • 1
    Souris
    Source
    • 5
    • 5
    • 5
    • 3
    • 1
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette CRLF2 protéine est marqué à la rho-1D4 tag.
    Application
    SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
    Séquence
    AAAVTSRGDV TVVCHDLETV EVTWGSGPDH HGANLSLEFR YGTGALQPCP RYFLSGAGVT SGCILPAARA GLLELALRDG GGAMVFKARQ RASAWLKPRP PWNVTLLWTP DGDVTVSWPA HSYLGLDYEV QHRESNDDED AWQTTSGPCC DLTVGGLDPA RCYDFRVRAS PRAAHYGLEA QPSEWTAVTR LSGAASAASC TASPAPSPAL APPLLPLGCG LAALLTLSLL LAALRLRRVK DALLPCVPDP SGSFPGLFEK HHGNFQAWIA DAQATAPPAR TEEEDDLIHP KAKRVEPEDG TSLCTVPRPP SFEPRGPGGG AMVSVGGATF MVGDSGYMTL
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Crlf2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin-free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product CRLF2 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    CRLF2 (Cytokine Receptor-Like Factor 2 (CRLF2))
    Autre désignation
    Crlf2 (CRLF2 Produits)
    Synonymes
    CRLF2 Protein, CRL2 Protein, CRLF2Y Protein, TSLPR Protein, CRLM2 Protein, Ly114 Protein, Tpte2 Protein, Tslpr Protein, Crl2 Protein, cytokine receptor like factor 2 Protein, cytokine receptor-like factor 2 Protein, CRLF2 Protein, Crlf2 Protein
    Sujet
    Receptor for thymic stromal lymphopoietin (TSLP). Forms a functional complex with TSLP and IL7R which is capable of stimulating cell proliferation through activation of STAT3 and STAT5. Also activates JAK2. Implicated in the development of the hematopoietic system. {ECO:0000269|PubMed:10974032}.
    Poids moléculaire
    37.0 kDa Including tag.
    UniProt
    Q8CII9
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