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TIMM50 Protein (AA 22-353) (rho-1D4 tag)

Crystallography grade TIMM50 Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
N° du produit ABIN3129294
  • Antigène Voir toutes TIMM50 Protéines
    TIMM50 (Translocase of Inner Mitochondrial Membrane 50 Homolog (TIMM50))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 22-353
    Origine
    • 3
    • 1
    Souris
    Source
    • 2
    • 1
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette TIMM50 protéine est marqué à la rho-1D4 tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Séquence
    LCTRLAPPPP RTPEQVTEIA NRGGSKAQGP QHQPGSEGPS YAKKIALWIA GLLGAGGTVS IVYIFGNNPV DENGTKIPDE FDSDPILVQQ LRRTYKYFKD YRQMIIEPTS PCLLPDPLRE PYYQPPYTLV LELTGVLLHP EWSLATGWRF KKRPGIETLF QQLAPLYEIV IFTSETGMTA FPLIDSVDPH GFISYRLFRD ATRYMEGHHV KDISCLNRDP ARVVVVDCKK EAFRLQPFNG VALRPWDGNS DDRVLLDLSA FLKTIALNQV EDVRTVLEHY ALEDDPLEAF KQRQSRLEQE EQQRLAELSK SNRQGLSFGS LASRLWPRSK QP
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Timm50 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin-free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product TIMM50 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    TIMM50 (Translocase of Inner Mitochondrial Membrane 50 Homolog (TIMM50))
    Autre désignation
    Timm50 (TIMM50 Produits)
    Synonymes
    F14J16.15 Protein, F14J16_15 Protein, emb1860 Protein, embryo defective 1860 Protein, 2810403L02Rik Protein, AU015082 Protein, TIM50L Protein, TIM50 Protein, Haloacid dehalogenase-like hydrolase (HAD) superfamily protein Protein, protein translocase subunit Protein, translocase of inner mitochondrial membrane 50 Protein, TIM50 Protein, Timm50 Protein, TIMM50 Protein
    Sujet
    Essential component of the TIM23 complex, a complex that mediates the translocation of transit peptide-containing proteins across the mitochondrial inner membrane. {ECO:0000250}.
    Poids moléculaire
    38.8 kDa Including tag.
    UniProt
    Q9D880
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