ELAPOR1 Protein (AA 36-1009) (rho-1D4 tag)

N° du produit ABIN3130738

Détail du produit

Antigène: ELAPOR1 (Endosome/Lysosome-associated Apoptosis and Autophagy Regulator 1 (ELAPOR1))

Type de proteíne: Recombinant

Attributs du protein: AA 36-1009

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette ELAPOR1 protéine est marqué à la rho-1D4 tag.

Application: Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: FQVTLGTGPE LHACKESEYH FEYTACDSTG SRWRVAVPHS PGLCTSLPDP VKGTECSFSC NAGEFLDMKD QSCKPCAEGR YSLGTGIRFD EWDELPHGFA SLSANLEVDD SISESTENCT SSKWVPRGDY IASNTDECTA TLMYAVNLKQ SGTVNFEYYY PDSSIIFEFF VQNDQCQPSA DDSRWMKTTE KGWVELNRGN NVLYWRTTAF SVWSKVSKPV LVRNIAITGV AYTSECFPCK PGTYAAKQGS PFCKLCPANY YSNKGETSCH PCDADKYSEK GSSTCKVRPA CTDKDYFYTH TACDAQGETQ LMYKWAIPKI CGEDLEGAVK LPASGVKTRC PPCNPGFFKT DNSTCEPCPY GSYSNGSDCT HCPAGTEPAV GFEYKWWNTL PSNMETTVLS GINFEYKGLT GWEVAGDHIY TAVGASDNDF MILTLVVPGF RPPQSVVADT ENKEVARITF VFETICSVNC ELYFMVGMNS RTNTPVETWK GTKGKQSYTY TIEENATVSF TWAFQRTTLH ETGRKYTNDV AKIYSINVTN VMGGVASYCR PCALEASDLG SSCTSCPAGH YINRDSGTCH LCPSNTILKA HQPYGAQACV PCGPGTKNNK IHSLCYNDCT FSRNTPSRIF NYNFSALAGT VSLAGVPSFT SKGLKYFHHF TLSLCGNQGK KMAVCTDNVT DLRIPDGEAG FSKSVTAYVC QVVIIPSEVM GYKAGVSSQP VSLADRLVGV STDMTLEGIV SPVELFHPET SGIPDIVFFF RSNDVTQSCS SGRSTTIRLR CNPMKAAPGT LRLPSMCSDG TCDGCNFHFL WESVAACPLC SASDYHTFVS SCVAGIQKTT YMWREPKLCS GGISLPEQRV TICKTIDFWL KVGISAGTCT AILLTVLTCY FWKKNQKLEY KYSKLVMNAT LKDCDLPAAD SCAIMEGEDV EDDFIFTSKK SLFGKIKSFT SKRTPDGFDS VPLKTSSGGP DMDM
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Kiaa1324 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin-free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: ELAPOR1 (Endosome/Lysosome-associated Apoptosis and Autophagy Regulator 1 (ELAPOR1))

Autre désignation: Kiaa1324 (ELAPOR1 Produits)

Synonymes: EIG121 Protein, BB183350 Protein, Kiaa1324 Protein, mKIAA1324 Protein, KIAA1324 Protein, RIKEN cDNA 5330417C22 gene Protein, KIAA1324 Protein, 5330417C22Rik Protein

Sujet: May protect cells from cell death by inducing cytosolic vacuolization and upregulating the autophagy pathway. {ECO:0000250}.

Poids moléculaire: 107.9 kDa Including tag.

UniProt: A2AFS3