Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

Catsperg2 Protein (AA 39-1145) (rho-1D4 tag)

Crystallography grade CATSPERG2 Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
N° du produit ABIN3131075
  • Antigène Tous les produits Catsperg2 (CATSPERG2)
    Catsperg2 (CATSPERG2) (Cation Channel, Sperm-Associated, gamma 2 (CATSPERG2))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 39-1145
    Origine
    Souris
    Source
    • 1
    Cellules d'insectes
    Purification/Conjugué
    Cette Catsperg2 protéine est marqué à la rho-1D4 tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Séquence
    EDALQHCTWL LVLNKFEKVG LHLSKDRFQD HEPIDTVAKV FQKLTDSPID PSENYLSFPY YLQINFSCPG QNIEELARKG HLMGMKPMVQ INYMYSVNFY RWEMENVQIL MEAAPMRSTG YCPAEAMCVL NWYTPMPFKN GSVVSSVDIY TNGIGPFVSK KRFYVNMNGF LKRDASGKSL FAIGYESLVL KSSHFRLSKS RPLWYTVNHA PVFILGGFYD EKSILFSDSN FQDYVLLELS IDSCWVGSFY CPILGFSATI HDAIATESTL FIRQNQLVYY FTGTYITLFD KSHGSSRWVR VLPSECIKRL CPVYASGNGS EYVLALTTGK NEGYIHIGTI TDGLVSFEMV PDGWSVCEKL PGKNCSIDWA TYIADERNLL LLVKIDSGQF YLVNFNTEFK TLNILYKIPE FIPEAKELDF LVLLDTVTYT NTPMTPKGLF FNTLNNMLYI WGNFILQSYN REEFIFLADF PKESTIKYMV NSFKGQMAVV TENEEIWYFL EGGYDVYQVV PSQGWETYHN LQKMQKSSFH SEDESLVSLF FEDGKLFQLV YLFDVGKERL VKRLLPVGTL MEYNLPKPFT VVNQGNYQAI SFTHTCPFKE IHLIDVPKKH HASRTESYVA LPPLVSESLG FHNNNTLAVY QGLVYYLLWL HSKYDKPYAD PVHDPTWRWW QHKTKDKDYF FYLFSNRLAA EGIYINMNAY QKLYNMSGDY GIPDLFFLDK GNWFTITVVL LSHQDTFTSS DSQGPTINVD KKLAIAVTIA DPECLSVTVT QDVLLNRNAV INKIKVIDKK RCSEQGMIGR NIKKTSMMLK VLGAPGNCIQ RTYLGGIIQG FKVVPIFIGC PPGKRLAFDV SYTIMHSEEI NKHYFDCVIK DAEMPCFLFR DLFQPFFLVQ DLVTGDSGSF LGSYVLKVVG GGRTLNTIRD YTEEEIFRYN SPLDTTNSLI WKTKVERTTE DKKFYIMSHE SPGVEWLCLE NSPCYDIIPQ SIYPPEFFFK LLVSNRGVDN STYCDYKLTF IVHIHGLPLS SKRTSFIVMV STSFFIALVV FYILFCLVWP HIVKAWVSLR WRINNIMASE SYYTYASSTA GFSLQSHSFE GPSRAGSKED NVQAKTA
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Catsperg2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin-free.
    Classe de qualité
    Crystallography grade
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    Catsperg2 (CATSPERG2) (Cation Channel, Sperm-Associated, gamma 2 (CATSPERG2))
    Autre désignation
    Catsperg2 (CATSPERG2 Produits)
    Synonymes
    1700067C01Rik Protein, CATSPERG Protein, cation channel sperm associated auxiliary subunit gamma 2 Protein, Catsperg2 Protein
    Sujet
    Probably involved in sperm cell hyperactivation via its association with CATSPER1. Sperm cell hyperactivation is needed for sperm motility which is essential late in the preparation of sperm for fertilization. {ECO:0000269|PubMed:19516020}.
    Poids moléculaire
    128.2 kDa Including tag.
    UniProt
    C6KI89
Vous êtes ici:
Support technique