NOTCH4 Protein (AA 1411-1964) (rho-1D4 tag)
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- Antigène Voir toutes NOTCH4 Protéines
- NOTCH4 (Notch 4 (NOTCH4))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1411-1964
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Origine
- Souris
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Source
- Cellules d'insectes
- Purification/Conjugué
- Cette NOTCH4 protéine est marqué à la rho-1D4 tag.
- Application
- SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), ELISA
- Séquence
- MAAVGALEPL LPGPLLAAHP QAGTRPSANQ LPWPILCSPV VGVLLLALGA LLVLQLIRRR RREHGALWLP PGFIRRPQTQ QAPHRRRPPL GEDNIGLKAL KPEAEVDEDG VAMCSGPEEG EAEETASASR CQLWPLNSGC GELPQAAMLT PPQECESEVL DVDTCGPDGV TPLMSAVFCG GVQSTTGASP QRLGLGNLEP WEPLLDRGAC PQAHTVGTGE TPLHLAARFS RPTAARRLLE AGANPNQPDR AGRTPLHTAV AADAREVCQL LLASRQTTVD ARTEDGTTPL MLAARLAVED LVEELIAARA DVGARDKRGK TALHWAAAVN NARAARSLLQ AGADKDAQDS REQTPLFLAA REGAVEVAQL LLELGAARGL RDQAGLAPGD VARQRSHWDL LTLLEGAGPT TQEARAHART TPGGGAAPRC RTLSAGARPR GGGACLQART WSVDLGARGG KVYARCRSRS GSCGGPTTRG RRFSAGSRGR RGARASQDDW PRDWVALEAC GSACSAPIPP PSLTPSPERG SPQVAWGLPV HQEIPLNSVV RNLNGSSGTE TSQVAPA
- Specificité
- C-terminal Rho-tag
- Attributs du produit
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- Made in Germany - from design to production - by highly experienced protein experts.
- Mouse Notch4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This made-to-order protein has already been successfully produced. Please let us know if you are interested in purchasing a smaller amount of this protein. We will check our stock and make you a customized quote in case we can provide this protein in a smaller amount..
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
- Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
- The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Stérilité
- 0.22 μm filtered
- niveau d'endotoxine
- Protein is endotoxin-free.
- Classe de qualité
- Crystallography grade
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Commentaires
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Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 20 mM Tris, pH 8.0; 300 mM NaCl; 2 mM DTT, 2 mM EDTA, 0.1% Triton X-100
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- NOTCH4 (Notch 4 (NOTCH4))
- Autre désignation
- Notch4 (NOTCH4 Produits)
- Synonymes
- INT3 Protein, NOTCH3 Protein, Int-3 Protein, Int3 Protein, N4 Protein, Notch4 Protein, notch 4 Protein, neurogenic locus notch homolog protein 4 Protein, NOTCH4 Protein, Notch4 Protein, LOC100712850 Protein
- Sujet
- Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity). May regulate branching morphogenesis in the developing vascular system. {ECO:0000250, ECO:0000269|PubMed:11344305}.
- Poids moléculaire
- 59.5 kDa Including tag.
- UniProt
- P31695
- Pathways
- Signalisation Notch
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