SLC19A1 Protein (AA 1-512) (Strep Tag)

N° du produit ABIN3134009

Détail du produit

Antigène: SLC19A1 (Solute Carrier Family 19 (Folate Transporter), Member 1 (SLC19A1))

Type de proteíne: Recombinant

Attributs du protein: AA 1-512

Origine: Souris

Source: Tobacco (Nicotiana tabacum)

Purification/Conjugué: Cette SLC19A1 protéine est marqué à la Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Séquence: MVPTGQVAEK QAYEEPRQDH ELKSWRCLVF YLCFFGFMAQ LRPGESFITP FLLERKFTKE QVTNEIIPML PYSHLAVLVP VFLLTDYLRY KPVLVLQCLS FVCVWLLLLL GTSVVHMQLM EVFYSVTMAA RIAYSSYIFS LVHPSRYQRM ASYSRAAVLL GVFISSVLGQ ALVTVGHIST YTLNCVSLGF ILFSLVLSLF LKRPKRSLFF NRSTLARGAL PCELDQMHPG PDRPETRKLD RMLGTCRDSF LVRMLSELVE NARQPQLRLW CLWWVFNSSG YYLITYYVHV LWRSTDSSLS YNGAVDAAST LLSAITSFSA GFLSIRWTLW SKLVIAGVIA IQASLVFCMF QIRDIWVCYV TFVLFRGAYQ FLVPIATFQI ASSLSKELCA LVFGINTFLA TALKTCITLV VSDKRGLGLQ VRDQFRIYFI YFLMLSITCF AWAGLDGLRY CQRGRHQPLA QAQELRSPLE TSVQAISLQD GDLRGPQPSA PQLLSEDGME DDRGDLRVEA KA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Attributs du produit: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

niveau d'endotoxine: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Commentaires:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: SLC19A1 (Solute Carrier Family 19 (Folate Transporter), Member 1 (SLC19A1))

Autre désignation: Slc19a1 (SLC19A1 Produits)

Synonymes: A1 Protein, MHCBFB Protein, PO-GA Protein, RECC1 Protein, RFC Protein, RFC140 Protein, CHMD Protein, FOLT Protein, IFC1 Protein, REFC Protein, RFC1 Protein, LOC100226745 Protein, AI323572 Protein, RFC-1 Protein, MTX1 Protein, XRFC Protein, chmd Protein, folt Protein, ifc1 Protein, refc Protein, rfc1 Protein, slc19a1 Protein, replication factor C subunit 1 Protein, solute carrier family 19 member 1 Protein, folate transporter 1 Protein, solute carrier family 19 (folate transporter), member 1 Protein, solute carrier family 19 (folate transporter), member 1 L homeolog Protein, RFC1 Protein, SLC19A1 Protein, LOC100226745 Protein, Slc19a1 Protein, slc19a1.L Protein

Sujet: Reduced folate transporter (Intestinal folate carrier 1) (IFC-1) (Plasma membrane folate antiporter SLC19A1) (Reduced folate carrier 1) (RFC-1) (RFC1) (Solute carrier family 19 member 1),FUNCTION: Antiporter that mediates the import of reduced folates (PubMed:8276792, PubMed:8664315, PubMed:9111015, PubMed:9748272). Mechanistically, acts as a secondary active transporter, which exports intracellular organic anions down their concentration gradients to facilitate the uptake of its substrates (By similarity). Has high affinity for N5-methyltetrahydrofolate, the predominant circulating form of folate (PubMed:8276792, PubMed:9111015). Also able to mediate the import of antifolate drug methotrexate (PubMed:8276792, PubMed:8664315, PubMed:9748272). 5-amino-4-imidazolecarboxamide riboside (AICAR), when phosphorylated to AICAR monophosphate, can serve as an organic anion for antiporter activity (By similarity). {ECO:0000250|UniProtKB:P41440, ECO:0000269|PubMed:8276792, ECO:0000269|PubMed:8664315, ECO:0000269|PubMed:9111015, ECO:0000269|PubMed:9748272}.

Poids moléculaire: 58.2 kDa

UniProt: P41438

Pathways: Dicarboxylic Acid Transport