SRPRB Protein (AA 1-269) (Strep Tag)
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- Antigène Voir toutes SRPRB Protéines
- SRPRB (Signal Recognition Particle Receptor, B Subunit (SRPRB))
- Type de proteíne
- Recombinant
- Attributs du protein
- AA 1-269
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Origine
- Souris
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Source
- Tobacco (Nicotiana tabacum)
- Purification/Conjugué
- Cette SRPRB protéine est marqué à la Strep Tag.
- Application
- ELISA, Western Blotting (WB), SDS-PAGE (SDS)
- Séquence
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MASANTRRVG DGAGGAFQPY LDSLRQELQQ RDPTLLSVAV ALLAVLLTLV FWKFIWSRKS SQRAVLFVGL CDSGKTLLFV RLLTGQYRDT QTSITDSSAI YKVNNNRGNS LTLIDLPGHE SLRFQLLDRF KSSARAVVFV VDSAAFQREV KDVAEFLYQV LIDSMALKNS PSLLIACNKQ DIAMAKSAKL IQQQLEKELN TLRVTRSAAP STLDSSSTAP AQLGKKGKEF EFSQLPLKVE FLECSAKGGR GDTGSADIQD LEKWLAKIA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us. - Attributs du produit
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
- These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
- We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):
- In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Pureté
- ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- niveau d'endotoxine
- Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)
- Classe de qualité
- Crystallography grade
- Top Product
- Discover our top product SRPRB Protéine
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- Indications d'application
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
- Commentaires
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! - Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.
- Conseil sur la manipulation
- Avoid repeated freeze-thaw cycles.
- Stock
- -80 °C
- Stockage commentaire
- Store at -80°C.
- Date de péremption
- Unlimited (if stored properly)
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- Antigène
- SRPRB (Signal Recognition Particle Receptor, B Subunit (SRPRB))
- Autre désignation
- Srprb (SRPRB Produits)
- Synonymes
- wu:fk57h10 Protein, zgc:111820 Protein, zgc:92746 Protein, apmcf1 Protein, APMCF1 Protein, AA409543 Protein, Ab2-417 Protein, Ba1-667 Protein, Cc1-8 Protein, signal recognition particle receptor, B subunit Protein, SRP receptor beta subunit S homeolog Protein, SRP receptor beta subunit Protein, SRPRB Protein, srprb Protein, srprb.S Protein, Srprb Protein
- Sujet
- Signal recognition particle receptor subunit beta (SR-beta),FUNCTION: Component of the SRP (signal recognition particle) receptor (PubMed:7844142). Ensures, in conjunction with the signal recognition particle, the correct targeting of the nascent secretory proteins to the endoplasmic reticulum membrane system (PubMed:7844142). May mediate the membrane association of SR (PubMed:7844142). {ECO:0000269|PubMed:7844142}.
- Poids moléculaire
- 29.6 kDa
- UniProt
- P47758
- Pathways
- ER-Nucleus Signaling
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