SLC8A1 Protein (AA 33-970) (rho-1D4 tag)

N° du produit ABIN3134654

Détail du produit

Antigène: SLC8A1 (Solute Carrier Family 8 (Sodium/calcium Exchanger), Member 1 (SLC8A1))

Type de proteíne: Recombinant

Attributs du protein: AA 33-970

Origine: Souris

Source: Cellules d'insectes

Purification/Conjugué: Cette SLC8A1 protéine est marqué à la rho-1D4 tag.

Application: Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Séquence: DTEAETGGNE TTECTGSYYC KKGVILPIWE PQDPSFGDKI ARATVYFVAM VYMFLGVSII ADRFMSSIEV ITSQEKEITI KKPNGETTKT TVRIWNETVS NLTLMALGSS APEILLSVIE VCGHNFTAGD LGPSTIVGSA AFNMFIIIAL CVYVVPDGET RKIKHLRVFF VTAAWSIFAY TWLYIILSVS SPGVVEVWEG LLTFFFFPIC VVFAWVADRR LLFYKYVYKR YRAGKQRGMI IEHEGDRPAS KTEIEMDGKV VNSHVDNFLD GALVLEVDER DQDDEEARRE MARILKELKQ KHPEKEIEQL IELANYQVLS QQQKSRAFYR IQATRLMTGA GNILKRHAAD QARKAVSMHE VNMEMAENDP VSKIFFEQGT YQCLENCGTV ALTIMRRGGD LSTTVFVDFR TEDGTANAAS DYEFTEGTVI FKPGETQKEI RVGIIDDDIF EEDENFLVHL SNVRVSSDVS EDGILESNHA SSIACLGSPS TATITIFDDD HAGIFTFEEP VTHVSESIGI MEVKVLRTSG ARGNVIIPYK TIEGTARGGG EDFEDTCGEP EFQNDEIVKT ISVKVIDDEE YEKNKTFFIE IGEPRLVEMS EKKALLLNEL GGFTLTGKEM YGQPIFRKVH ARDHPIPSTV ITISEEYDDK QPLTSKEEEE RRIAEMGRPI LGEHTKLEVI IQESYEFKST VDKLIKKTNL ALVVGTNSWR EQFIEAITVS AGEDDDDDEC GEEKLPSCFD YVMHFLTVFW KVLFAFVPPT EYWNGWACFI VSILMIGLLT AFIGDLASHF GCTIGLKDSV TAVVFVALGT SVPDTFASKV AATQDQYADA SIGNVTGSNA VNVFLGIGVA WSIAAIYHAA NGEQFKVSPG TLAFSVTLFT IFAFINVGVL LYRRRPEIGG ELGGPRTAKL LTSSLFVLLW LLYIFFSSLE AYCHIKGF
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Attributs du produit:

• Made in Germany - from design to production - by highly experienced protein experts.
• Mouse Slc8a1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Pureté: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Stérilité: 0.22 μm filtered

niveau d'endotoxine: Protein is endotoxin-free.

Classe de qualité: Crystallography grade

Information d'application

Indications d'application: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Commentaires:

Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Conseil sur la manipulation: Avoid repeated freeze-thaw cycles.

Stock: -80 °C

Stockage commentaire: Store at -80°C.

Date de péremption: Unlimited (if stored properly)

Détail du antigène

Antigène: SLC8A1 (Solute Carrier Family 8 (Sodium/calcium Exchanger), Member 1 (SLC8A1))

Autre désignation: Slc8a1 (SLC8A1 Produits)

Synonymes: NCX1 Protein, AI852629 Protein, AV344025 Protein, D930008O12Rik Protein, Ncx1 Protein, Ncx Protein, SLC8A1 Protein, ncx1n Protein, solute carrier family 8 member A1 Protein, solute carrier family 8 (sodium/calcium exchanger), member 1 Protein, solute carrier family 8 (sodium/calcium exchanger), member 1b Protein, sodium/calcium exchanger 1 Protein, SLC8A1 Protein, Slc8a1 Protein, slc8a1b Protein, Tsp_07038 Protein

Sujet: Mediates the exchange of one Ca(2+) ion against three to four Na(+) ions across the cell membrane, and thereby contributes to the regulation of cytoplasmic Ca(2+) levels and Ca(2+)-dependent cellular processes (PubMed:8659820). Contributes to Ca(2+) transport during excitation-contraction coupling in muscle. In a first phase, voltage-gated channels mediate the rapid increase of cytoplasmic Ca(2+) levels due to release of Ca(2+) stores from the endoplasmic reticulum. SLC8A1 mediates the export of Ca(2+) from the cell during the next phase, so that cytoplasmic Ca(2+) levels rapidly return to baseline (PubMed:10967099). Required for normal embryonic heart development and the onset of heart contractions (PubMed:10967099). {ECO:0000269|PubMed:10967099, ECO:0000269|PubMed:8659820, ECO:0000305}.

Poids moléculaire: 105.7 kDa Including tag.

UniProt: P70414

Pathways: Myometrial Relaxation and Contraction