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TRPM1 Protein (AA 1-1622) (rho-1D4 tag)

Crystallography grade TRPM1 Origine: Souris Hôte: Cellules d'insectes Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, Crys, ELISA
N° du produit ABIN3134946
  • Antigène Voir toutes TRPM1 Protéines
    TRPM1 (Transient Receptor Potential Cation Channel, Subfamily M, Member 1 (TRPM1))
    Type de proteíne
    Recombinant
    Attributs du protein
    AA 1-1622
    Origine
    • 5
    • 2
    Souris
    Source
    • 5
    • 2
    Cellules d'insectes
    Purification/Conjugué
    Cette TRPM1 protéine est marqué à la rho-1D4 tag.
    Application
    SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), ELISA
    Séquence
    MGSMRKMSSS FKRGSIKSST SGSQKGQKAW IEKTFCKREC IFVIPSTKDP NRCCCGQLTN QHIPPLPSGA PSTTGEDTKQ ADTQSGKWSV SKHTQSYPTD SYGILEFQGG GYSNKAMYIR VSYDTKPDSL LHLMVKDWQL ELPKLLISVH GGLQSFEMQP KLKQVFGKGL IKAAMTTGAW IFTGGVSTGV VSHVGDALKD HSSKSRGRLC AIGIAPWGMV ENKEDLIGKD VTRVYQTMSN PLSKLSVLNN SHTHFILADN GTLGKYGAEV KLRRQLEKHI SLQKINTRLG QGVPVVGLVV EGGPNVVSIV LEYLKEDPPV PVVVCDGSGR ASDILSFAHK YCDEGGVINE SLRDQLLVTI QKTFNYSKSQ SYQLFAIIME CMKKKELVTV FRMGSEGQQD VEMAILTALL KGTNASAPDQ LSLALAWNRV DIARSQIFVF GPHWPPLGSL APPVDTKATE KEKKPPTATT KGRGKGKGKK KGKVKEEVEE ETDPRKLELL NWVNALEQAM LDALVLDRVD FVKLLIENGV NMQHFLTIPR LEELYNTRLG PPNTLHLLVR DVKKSNLPPD YHISLIDIGL VLEYLMGGAY RCNYTRKSFR TLYNNLFGPK RPKALKLLGM EDDEPPAKGK KKKKKKKEEE IDIDVDDPAV SRFQYPFHEL MVWAVLMKRQ KMAVFLWQRG EECMAKALVA CKLYKAMAHE SSESELVDDI SQDLDNNSKD FGQLAVELLD QSYKHDEQVA MKLLTYELKN WSNSTCLKLA VAAKHRDFIA HTCSQMLLTD MWMGRLRMRK NPGLKVIMGI LIPPTILFLE FRTYDDFSYQ TSKENEDGKE KEEENVDANA DAGSRKGDEE NEHKKQRSIP IGTKICEFYN APIVKFWFYT ISYLGYLLLF NYVILVRMDG WPSPQEWIVI SYIVSLALEK IREILMSEPG KLSQKIKVWL QEYWNITDLV AISMFMVGAI LRLQSQPYMG YGRVIYCVDI ILWYIRVLDI FGVNKYLGPY VMMIGKMMID MLYFVVIMLV VLMSFGVARQ AILHPEEKPS WKLARNIFYM PYWMIYGEVF ADQIDLYAME INPPCGENLY DEEGKRLPPC IPGAWLTPAL MACYLLVANI LLVNLLIAVF NNTFFEVKSI SNQVWKFQRY QLIMTFHDRP VLPPPMIILS HIYIIIMRLS GRCRKKREGD QEERDRGLKL FLSDEELKKL HEFEEQCVQE HFREKEDEQQ SSSDERIRVT SERVENMSMR LEEINERENF MKTSLQTVDL RLSQLEELSG RMVSALENLA GIDRSDLIQA RSRASSECEA TYLLRQSSIN SADGYSLYRY HFNGEELLFE EPALSTSPGT AFRKKTYSFR VKDEDAKSHL DQPSNLHHTP GPSPPATPGR SRLALEGPLS TELRPGSDPG ISAGEFDPRA DFKSTEAAPS LNAAGVTGTQ LTVESTDSHP LRESKLVRYY PGDPNTYKTM KSRSFVYTEG RKLVRGLSNW SAEYSSIMDQ AWNATEWRCQ VQRITRSRST DIPYIVSEAA SQDELEDEHR GSLLDPQISR SALTVSDRPE KENLLSVKPH QTLGFPCLRS RSLHGRPRSA EPAPSKLDRA GHASSTSNLA VMSVVPEGQN TQQEKRSAET EC
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Attributs du produit
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Mouse Trpm1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Pureté
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Stérilité
    0.22 μm filtered
    niveau d'endotoxine
    Protein is endotoxin-free.
    Classe de qualité
    Crystallography grade
    Top Product
    Discover our top product TRPM1 Protéine
  • Indications d'application
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Commentaires

    Protein has not been tested for activity yet. In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Conseil sur la manipulation
    Avoid repeated freeze-thaw cycles.
    Stock
    -80 °C
    Stockage commentaire
    Store at -80°C.
    Date de péremption
    Unlimited (if stored properly)
  • Antigène
    TRPM1 (Transient Receptor Potential Cation Channel, Subfamily M, Member 1 (TRPM1))
    Autre désignation
    Trpm1 (TRPM1 Produits)
    Synonymes
    TRPM1b Protein, trpm1 Protein, zgc:158632 Protein, CSNB1C Protein, LTRPC1 Protein, MLSN1 Protein, 4732499L03Rik Protein, AI606771 Protein, Ltrpc1 Protein, Mlsn1 Protein, melastatin Protein, transient receptor potential cation channel subfamily M member 1 Protein, transient receptor potential cation channel, subfamily M, member 1a Protein, transient receptor potential cation channel, subfamily M, member 1 Protein, TRPM1 Protein, trpm1a Protein, LOC100541416 Protein, trpm1 Protein, Trpm1 Protein
    Sujet
    Cation channel essential for the depolarizing photoresponse of retinal ON bipolar cells. It is part of the GRM6 signaling cascade. Calcium channel which may play a role in metastasis suppression. May act as a spontaneously active, calcium-permeable plasma membrane channel. {ECO:0000269|PubMed:19861548, ECO:0000269|PubMed:19966281, ECO:0000269|PubMed:9537257}.
    Poids moléculaire
    184.7 kDa Including tag.
    UniProt
    Q2TV84
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